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PMID: 2828307 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Confirmation of the Fur operator site by insertion of a synthetic oligonucleotide into an operon fusion plasmid.

Journal of bacteriology ·Vol. 170 ·No. 2 ·1988-02-00 ·Pages 1015-7

Calderwood SB, Mekalanos JJ

Abstract

We constructed a synthetic oligonucleotide corresponding to the previously proposed consensus binding site for the Fur protein, a central iron-regulatory protein of Escherichia coli. When this oligonucleotide was introduced at the start of transcription of an operon fusion between the ompF promoter and the lacZ structural gene, beta-galactosidase activity became iron regulated. This consensus sequence is sufficient to function as an operator site for the binding of Fur protein in vivo.

MeSH Terms
Bacterial Proteins/genetics,metabolism Base Sequence Cloning, Molecular Escherichia coli/genetics Genes, Bacterial Iron/metabolism Oligodeoxyribonucleotides Operator Regions, Genetic Operon Plasmids beta-Galactosidase/analysis
Chemicals
Bacterial Proteins Oligodeoxyribonucleotides Iron beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Calderwood S B
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Mekalanos J J
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16 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.2.1015-1017.1988
Published
1988-02-00
Pages
1015-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210760
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.170.2.1015-1017.1988 PMC Full Text View journal details
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