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PMID: 2828888 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 210 ·No. 1 ·1987-11-00 ·Pages 116-21

Jannière L, Ehrlich SD

Abstract

We measured in Bacillus subtilis the recombination frequency between directly repeated 9 bp sequences flanking the transposon Tn5. Four different repeats were analyzed. They recombined with frequencies between 10(-7) and 10(-6) when they were carried on plasmids, and 150-1500 times less frequently when they were carried on the chromosome. We propose that this difference is due to the generation of single stranded DNA during plasmid replication.

MeSH Terms
Bacillus subtilis/genetics Chromosomes, Bacterial DNA Replication DNA Transposable Elements DNA, Bacterial/genetics DNA, Single-Stranded/genetics Plasmids Recombination, Genetic Repetitive Sequences, Nucleic Acid
Chemicals
DNA Transposable Elements DNA, Bacterial DNA, Single-Stranded
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jannière L
Institut Jacques Monod, Paris, France.
Ehrlich S D
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33 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00337766
Published
1987-11-00
Pages
116-21
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00337766
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