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PMID: 6439976 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Recombination between repeated DNA sequences occurs more often in plasmids than in the chromosome of Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 197 ·No. 1 ·1984-00-00 ·Pages 46-54

Niaudet B, Jannière L, Ehrlich SD

Abstract

Directly repeated pBR322 sequences 3.7-3.8 kb long recombine with a frequency of about 10% per generation when carried on plasmids related to pC194 and 0.01% per generation when carried on the Bacillus subtilis chromosome. Recombination is therefore 1,000 times more efficient in plasmids than in the chromosome of this organism.

MeSH Terms
Bacillus subtilis/genetics Chromosomes, Bacterial/metabolism DNA, Bacterial/genetics Nucleic Acid Hybridization Plasmids Recombination, Genetic Repetitive Sequences, Nucleic Acid Transformation, Genetic
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Niaudet B
Jannière L
Ehrlich S D
References (38)
38 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. Random replication and random assortment model for plasmid incompatibility in bacteria.
    Plasmid. 1978 Sep;1(4):435-45 PMID: 748953
  3. Rapid purification of plasmid DNAs by hydroxyapatite chromatography.
    Eur J Biochem. 1978 Nov 2;91(1):303-10 PMID: 363426
  4. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  5. Isolation of plasmid deletion Mutants and study of their instability.
    Plasmid. 1981 Sep;6(2):193-201 PMID: 7029580
  6. The conjugation system of F-like plasmids.
    Annu Rev Genet. 1980;14:41-76 PMID: 6111288
  7. Intramolecular recombination during plasmid transformation of Bacillus subtilis competent cells.
    EMBO J. 1982;1(12):1565-71 PMID: 6327279
  8. In vitro genetic labeling of Bacillus subtilis cryptic plasmid pHV400.
    Plasmid. 1979 Jan;2(1):48-58 PMID: 112601
  9. Study of the phenomenon of marker rescue in plasmid transformation and transduction of intact cells, protoplasts and different rec mutants of Bacillus subtilis.
    Mol Gen Genet. 1982;185(2):363-4 PMID: 6806568
  10. Intermolecular recombination during transformation of Bacillus subtilis competent cells by monomeric and dimeric plasmids.
    Plasmid. 1983 Jul;10(1):1-10 PMID: 6413989
  11. Recombination between compatible plasmids containing homologous segments requires the Bacillus subtilis recE gene product.
    J Bacteriol. 1978 May;134(2):514-20 PMID: 96091
  12. recE4-Independent recombination between homologous deoxyribonucleic acid segments of Bacillus subtilis plasmids.
    J Bacteriol. 1979 Sep;139(3):775-82 PMID: 113386
  13. Specialized transduction with lambda plac5: dependence on recB.
    J Bacteriol. 1982 Jun;150(3):1485-8 PMID: 6210690
  14. Genetic recombination of bacterial plasmid DNA. Analysis of the effect of recombination-deficient mutations on plasmid recombination.
    J Mol Biol. 1982 Sep 25;160(3):411-30 PMID: 6759658
  15. Recombination deficient mutants of E. coli and other bacteria.
    Annu Rev Genet. 1973;7:67-86 PMID: 4205905
  16. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  17. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance.
    J Bacteriol. 1982 May;150(2):815-25 PMID: 6950931
  18. Replication functions of pC194 are necessary for efficient plasmid transduction by M13 phage.
    EMBO J. 1984 Jan;3(1):81-6 PMID: 6323171
  19. Insertional mutagenesis in Bacillus subtilis: mechanism and use in gene cloning.
    Gene. 1982 Oct;19(3):277-84 PMID: 6295881
  20. Electrophoretic separation of Bacillus subtilis genes.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2207-11 PMID: 806078
  21. On plasmid incompatibility.
    Plasmid. 1978 Sep;1(4):421-34 PMID: 372974
  22. DNA recombination: in vivo and in vitro studies.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:969-85 PMID: 385237
  23. Interplasmidic and intraplasmidic recombination in Escherichia coli K-12.
    Mol Gen Genet. 1981;184(2):200-7 PMID: 7035834
  24. Recombination between bacterial plasmids leading to the formation of plasmid multimers.
    Cell. 1976 Dec;9(4 PT 2):707-16 PMID: 797459
  25. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  26. Recombination properties of P1 dlac.
    J Bacteriol. 1982 Oct;152(1):345-50 PMID: 6811556
  27. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.
    J Bacteriol. 1978 Apr;134(1):318-29 PMID: 418061
  28. Prophage P1, and extrachromosomal replication unit.
    Cold Spring Harb Symp Quant Biol. 1968;33:791-8 PMID: 4892009
  29. Plasmid marker rescue transformation in Bacillus subtilis.
    J Bacteriol. 1983 Jun;154(3):1077-87 PMID: 6406424
  30. Enhanced recombination between F42lac and lambda plac5: dependence on F42lac fertility functions.
    Mol Gen Genet. 1981;184(3):355-8 PMID: 6801433
  31. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  32. Complete nucleotide sequence of the Escherichia coli plasmid pBR322.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:77-90 PMID: 383387
  33. Special sites in generalized recombination.
    Annu Rev Genet. 1979;13:7-24 PMID: 395896
  34. Isolation of genetic elements that increase frequencies of plasmid recombinants.
    Nature. 1983 May 19-25;303(5914):256-9 PMID: 6341860
  35. Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: phenotypic characterization of radiation-sensitive recombination-deficient mutants.
    J Bacteriol. 1973 Apr;114(1):273-86 PMID: 4349031
  36. Transduction versus "conjuduction": evidence for multiple roles for exonuclease V in genetic recombination in Escherichia coli.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1043-7 PMID: 158457
  37. recA-independent general genetic recombination of plasmids.
    Nature. 1981 Nov 12;294(5837):184-6 PMID: 7029308
  38. Mapping a cloned gene under sporulation control by inserttion of a drug resistance marker into the Bacillus subtilis chromosome.
    J Bacteriol. 1980 Apr;142(1):90-8 PMID: 6768719
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00327921
Published
1984-00-00
Pages
46-54
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00327921
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