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PMID: 7035834 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Interplasmidic and intraplasmidic recombination in Escherichia coli K-12.

Molecular & general genetics : MGG ·Vol. 184 ·No. 2 ·1981-00-00 ·Pages 200-7

Laban A, Cohen A

Abstract

The construction of plasmids which facilitate the study of interplasmidic and intraplasmidic recombination is described. In this system, a single recombination event between two mutated Tcr genes on separate plasmids or on one plasmid leads to a change in the host phenotype from sensitivity to resistance to tetracycline. Recombination proficiencies have been determined for different E. coli K-12 strains: both interplasmidic and intraplasmidic recombination are independent of the recBC gene product. RecA mutations decrease the proficiency of plasmidic recombination 40 - 100 fold. Intraplasmidic and interplasmidic recombination via the recE pathway are more efficient than via the recBC pathway. Intraplasmidic recombination, but not interplasmidic recombination via the recE pathway is independent of a functional recA product.

MeSH Terms
Escherichia coli/genetics Mutation Plasmids Recombination, Genetic Species Specificity
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Laban A
Cohen A
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31 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00272905
Published
1981-00-00
Pages
200-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00272905
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