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PMID: 325373 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Construction and restriction endonuclease mapping of hybrid plasmids containing Saccharomyces cerevisiae ribosomal DNA.

Molecular & general genetics : MGG ·Vol. 151 ·No. 3 ·1977-03-16 ·Pages 229-44

Cramer JH, Farrelly FW, Barnitz JT, Rownd RH

Abstract

Fragments produced by partial digestion of Saccharomyces cerevisiae ribosomal DNA (rDNA) with the restriction endonuclease EcoRI were ligated in vitro to the bacterial plasmid RSF2124. The resulting hybrid plasmids were cloned in Escherichia coli. Three hybrid plasmids which contain at least one intact repetitive unit of the multiple, tandem sequences of the yeast rDNA genes have been further characterized. These plasmids have been used to construct a map of the EcoRI, SmaI, HindII and HindIII restriction sites in the individual repetitive units of yeast rDNA.

MeSH Terms
DNA DNA Restriction Enzymes DNA, Recombinant Electrophoresis, Agar Gel Escherichia coli Extrachromosomal Inheritance Plasmids RNA, Ribosomal/biosynthesis Saccharomyces cerevisiae
Chemicals
DNA, Recombinant RNA, Ribosomal DNA DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cramer J H
Farrelly F W
Barnitz J T
Rownd R H
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22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268786
Published
1977-03-16
Pages
229-44
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268786
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