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PMID: 2829119 Published · ppublish English Journal Article

DNA containing the base analogue 2-aminoadenine: preparation, use as hybridization probes and cleavage by restriction endonucleases.

Nucleic acids research ·Vol. 16 ·No. 1 ·1988-01-11 ·Pages 305-17

Chollet A, Kawashima E

Abstract

The base analogue 2-aminoadenine (2,6-diaminopurine, D) has been introduced at selected positions into synthetic oligodeoxyribonucleotides and DNA by the combined use of chemical and enzymatic methods. 2-aminoadenine substitution for adenine introduces changes in the minor groove of DNA and creates an additional hydrogen bond in the Watson-Crick base pair with thymine. Oligonucleotide hybridization probes containing 2-aminoadenine showed increased selectivity and hybridization strength during DNA-DNA hybridization to phage or genomic target DNA. Properties of the base analogue with respect to DNA modifying enzymes were examined. 2-aminoadenine was used to probe minor groove determinants during the treatment of DNA by 12 restriction endonucleases. Inhibition of cleavage was found for several restriction enzymes.

MeSH Terms
2-Aminopurine/analogs & derivatives Adenine/analogs & derivatives Base Sequence DNA/chemical synthesis DNA Restriction Enzymes Indicators and Reagents Molecular Sequence Data Nucleic Acid Hybridization Oligodeoxyribonucleotides/chemical synthesis Templates, Genetic
Chemicals
Indicators and Reagents Oligodeoxyribonucleotides 2-Aminopurine 2,6-diaminopurine DNA DNA Restriction Enzymes Adenine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chollet A
Department of Chemistry, Biogen SA, Geneva, Switzerland.
Kawashima E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.1.305
Published
1988-01-11
Pages
305-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334628
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.1.305 PMC Full Text View journal details
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