Home LiteratureArticle Details
PMID: 6280151 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Digestion of highly modified bacteriophage DNA by restriction endonucleases.

Nucleic acids research ·Vol. 10 ·No. 5 ·1982-03-11 ·Pages 1579-91

Huang LH, Farnet CM, Ehrlich KC, Ehrlich M

Abstract

The ability of thirty Type II restriction endonucleases to cleave five different types of highly modified DNA has been examined. The DNA substrates were derived from relatively large bacteriophage genomes which contain all or most of the cytosine or thymine residues substituted at the 5-position. These substituents were a proton (PBS1 DNA), a hydroxymethyl group (SP01 DNA), a methyl group (XP12 DNA), a glucosylated hydroxymethyl group (T4 DNA), or a phosphoglucuronated, glucosylated 4,5-dihydroxypentyl group (SP15 DNA). Although PBS1 DNA and SP01 DNA were digested by most of the enzymes, they were cleaved much more slowly than was normal DNA by many of them. 5-Methylcytosine-rich XP12 DNA and the multiply modified T4 and SP15 DNAs were resistant to most of these endonucleases. The only enzyme that cleaved all five of these DNAs was TaqI, which fragmented them extensively.

MeSH Terms
Base Sequence DNA Restriction Enzymes/metabolism DNA, Viral Deoxyribonuclease I Deoxyribonucleases Endonucleases Kinetics Phosphoric Diester Hydrolases Substrate Specificity
Chemicals
DNA, Viral Deoxyribonucleases Endonucleases DNA Restriction Enzymes Deoxyribonuclease I Phosphoric Diester Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Huang L H
Farnet C M
Ehrlich K C
Ehrlich M
References (43)
43 references, click to expand
  1. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  2. Cotransduction and cotransformation of genetic markers in Bacillus subtilis and Bacillus licheniformis.
    J Bacteriol. 1969 Nov;100(2):1027-36 PMID: 4982666
  3. [Comparative study of the DNA of phages 2C, SP8*, SP82, phi e, SP01 and SP50].
    Eur J Biochem. 1970 Aug;15(2):391-400 PMID: 4993758
  4. 5-Methylcytosine replacing cytosine in the deoxyribonucleic acid of a bacteriophage for Xanthomonas oryzae.
    J Mol Biol. 1968 Jul 14;34(2):373-5 PMID: 5760463
  5. Structure and synthesis of dihydroxypentyluracil from bacteriophage SP-15 deoxyribonucleic acid.
    J Am Chem Soc. 1973 Dec 26;95(26):8749-57 PMID: 4783412
  6. Cleavage of Nonglucosylated Bacteriophage T4 deoxyribonucleic acid by Restriction Endonuclease Eco RI.
    J Biol Chem. 1975 Mar 25;250(6):2395-7 PMID: 1090619
  7. Nature of R-factor replication in the presence of chloramphenicol.
    Proc Natl Acad Sci U S A. 1975 Feb;72(2):654-8 PMID: 1091928
  8. Unusual properties of the DNA from Xanthomonas phage XP-12 in which 5-methylcytosine completely replaces cytosine.
    Biochim Biophys Acta. 1975 Jun 16;395(2):109-19 PMID: 1138935
  9. Bacteriophages of Bacillus subtilis.
    Bacteriol Rev. 1975 Sep;39(3):257-315 PMID: 809034
  10. The sensitivity of bacteriophage lambda DNA to restriction endonuclease RII.
    J Mol Biol. 1975 Nov 5;98(3):645-7 PMID: 1104876
  11. Sequence-specific recognition of double helical nucleic acids by proteins.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):804-8 PMID: 1062791
  12. Specificity of Hpa II and Hae III DNA methylases.
    Nucleic Acids Res. 1977 Dec;4(12):4211-21 PMID: 600794
  13. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  14. Sequence specificity of the wild-type dam+) and mutant (damh) forms of bacteriophage T2 DNA adenine methylase.
    J Mol Biol. 1978 Mar 5;119(3):361-76 PMID: 641992
  15. EcoRI cleavage of DNA from Bacillus subtilis phage SPO1.
    Virology. 1978 Apr;85(2):601-5 PMID: 96586
  16. MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.
    Nucleic Acids Res. 1978 Sep;5(9):3231-6 PMID: 704354
  17. The effects of substituted pyrimidines in DNAs on cleavage by sequence-specific endonucleases.
    J Biol Chem. 1979 Apr 10;254(7):2551-60 PMID: 372183
  18. Nucleotide sequence specificity of restriction endonucleases.
    Science. 1979 Aug 3;205(4405):455-62 PMID: 377492
  19. Restriction cleavage map of SP01 DNA: general location of early, middle, and late genes.
    J Virol. 1979 Jul;31(1):156-71 PMID: 116010
  20. In vivo methylation of bacteriophage phi X174 DNA.
    J Virol. 1979 Dec;32(3):845-51 PMID: 159962
  21. Solvent-accessible surfaces of nucleic acids.
    J Mol Biol. 1979 Aug 15;132(3):411-34 PMID: 533898
  22. Two sequence-specific endonucleases from Xanthomonas oryzae. Characterization and unusual properties.
    Biochim Biophys Acta. 1980 Feb 29;606(2):371-85 PMID: 6243986
  23. Structures and mechanisms of DNA restriction and modification enzymes.
    Q Rev Biophys. 1979 Aug;12(3):315-69 PMID: 232555
  24. Isolation and characterization of DNA fragments containing the dihydrofolate-reductase gene of coliphage T4.
    Gene. 1980 May;9(3-4):271-85 PMID: 6993288
  25. Non-specific binding of restriction endonuclease EcoR1 to DNA.
    Nucleic Acids Res. 1980 Jan 25;8(2):389-402 PMID: 6252547
  26. Modified bases in bacteriophage DNAs.
    Annu Rev Microbiol. 1980;34:137-58 PMID: 7002022
  27. Polymorphism of DNA double helices.
    J Mol Biol. 1980 Oct 15;143(1):49-72 PMID: 7441761
  28. Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA.
    Nucleic Acids Res. 1980 Oct 24;8(20):4763-76 PMID: 7003544
  29. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1981 Jan 10;9(1):r75-96 PMID: 6259615
  30. Enzymatic synthesis, ligation, and restriction of DNA containing deoxy-4-thiothymidine.
    Nucleic Acids Res. 1981 Feb 25;9(4):753-67 PMID: 6262716
  31. 5-Methylcytosine in eukaryotic DNA.
    Science. 1981 Jun 19;212(4501):1350-7 PMID: 6262918
  32. Map of restriction sites on bacteriophage T4 cytosine-containing DNA for endonucleases bamHI, BglII, KpnI, PvuI, SalI, and XbaI.
    J Virol. 1981 Apr;38(1):104-14 PMID: 6264096
  33. Single-strand and double-strand cleavage at half-modified and fully modified recognition sites for the restriction nucleases Sau3a and Taqi.
    Gene. 1980 Dec;12(3-4):267-75 PMID: 6265320
  34. Restriction and modification in Bacillus subtilis: two DNA methyltransferases with BsuRI specificity. I. Purification and physical properties.
    J Biol Chem. 1981 Sep 10;256(17):9340-5 PMID: 6267072
  35. A novel, highly modified, bacteriophage DNA in which thymine is partly replaced by a phosphoglucuronate moiety covalently bound to 5-(4',5'-dihydroxypentyl)uracil.
    J Biol Chem. 1981 Oct 10;256(19):9966-72 PMID: 6268637
  36. Restriction enzyme digestion of hemimethylated DNA.
    Nucleic Acids Res. 1981 Jun 11;9(11):2509-15 PMID: 6269052
  37. DNA methyltransferases affecting the sequence 5'CCGG.
    Nucleic Acids Res. 1981 Jun 25;9(12):2753-9 PMID: 6269059
  38. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  39. rII cistrons of bacteriophage T4. DNA sequence around the intercistronic divide and positions of genetic landmarks.
    J Mol Biol. 1981 Jul 5;149(3):337-76 PMID: 6273585
  40. Sequence-dependent variation in the conformation of DNA.
    J Mol Biol. 1981 Jul 15;149(4):745-60 PMID: 6273590
  41. On the structure of the glucosylated hydroxymethylcytosine nucleotides of coliphages T2, T4, and T6.
    J Biol Chem. 1960 Nov;235:3254-9 PMID: 13760441
  42. The new occurrence of a new pyrimidine base replacing thymine in a bacteriophage DNA:5-hydroxymethyl uracil.
    J Mol Biol. 1962 Aug;5:248-50 PMID: 13961966
  43. Replacement of thymidylic acid by deoxyuridylic acid in the deoxyribonucleic acid of a transducing phage for Bacillus subtilis.
    Nature. 1963 Feb 23;197:794-5 PMID: 13980287
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.5.1579
Published
1982-03-11
Pages
1579-91
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320551
Subset
IM
Grants
NCI NIH HHS · CA-19942 · United States
NIGMS NIH HHS · GM-26986 · United States
External Links
PubMed source DOI: 10.1093/nar/10.5.1579 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2