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PMID: 2829842 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Guanine-nucleotide and hormone regulation of polyphosphoinositide phospholipase C activity of rat liver plasma membranes. Bivalent-cation and phospholipid requirements.

The Biochemical journal ·Vol. 248 ·No. 3 ·1987-12-15 ·Pages 791-9

Taylor SJ, Exton JH

Abstract

The effect of the GTP analogue guanosine 5'-[gamma-thio]triphosphate (GTP[S]) on the polyphosphoinositide phospholipase C (PLC) of rat liver was examined by using exogenous [3H]phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2]. GTP[S] stimulated the membrane-bound PLC up to 20-fold, with a half-maximal effect at approx. 100 nM. Stimulation was also observed with guanosine 5'-[beta gamma-imido]triphosphate, but not with adenosine 5'-[gamma-thio]triphosphate, and was inhibited by guanosine 5'-[beta-thio]diphosphate. Membrane-bound PLC was entirely Ca2+-dependent, and GTP[S] produced both a decrease in the Ca2+ requirement and an increase in activity at saturating [Ca2+]. The stimulatory action of GTP[S] required millimolar Mg2+. [8-arginine]Vasopressin (100 nM) stimulated the PLC activity approx. 2-fold in the presence of 10 nM-GTP[S], but had no effect in the absence of GTP[S] or at 1 microM-GTP[S]. The hydrolysis of PtdIns(4,5)P2 by membrane-bound PLC was increased when the substrate was mixed with phosphatidylethanolamine, phosphatidylcholine or various combinations of these with phosphatidylserine. With PtdIns(4,5)P2, alone or mixed with phosphatidylcholine, GTP[S] evoked little or no stimulation of the PLC activity. However, maximal stimulation by GTP[S] was observed in the presence of a 2-fold molar excess of phosphatidylserine or various combinations of phosphatidylethanolamine and phosphatidylserine. Hydrolysis of [3H]phosphatidylinositol 4-phosphate by membrane-bound PLC was also increased by GTP[S]. However, [3H]phosphatidylinositol was a poor substrate, and its hydrolysis was barely affected by GTP[S]. Cytosolic PtdIns(4,5)P2-PLC exhibited a Ca2+-dependence similar to that of the membrane-bound activity, but was unaffected by GTP[S]. It is concluded that rat liver plasma membranes possess a Ca2+-dependent polyphosphoinositide PLC that is activated by hormones and GTP analogues, depending on the Mg2+ concentration and phospholipid environment. It is proposed that GTP analogues and hormones, acting through a guanine nucleotide-binding protein, activate the enzyme mainly by lowering its Ca2+ requirement.

MeSH Terms
Adenosine Triphosphate/pharmacology Animals Arginine Vasopressin/pharmacology Calcium/pharmacology Cell Membrane/drug effects,enzymology Guanine Nucleotides/pharmacology Liver/drug effects,enzymology Magnesium/pharmacology Phosphatidylinositol Phosphates Phosphatidylinositols/metabolism Phosphoinositide Phospholipase C Phospholipids/pharmacology Phosphoric Diester Hydrolases/metabolism Rats Substrate Specificity
Chemicals
Guanine Nucleotides Phosphatidylinositol Phosphates Phosphatidylinositols Phospholipids Arginine Vasopressin Adenosine Triphosphate Phosphoric Diester Hydrolases Phosphoinositide Phospholipase C Magnesium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Taylor S J
Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232.
Exton J H
References (42)
42 references, click to expand
  1. Guanine nucleotide-binding regulatory proteins and dual control of adenylate cyclase.
    J Clin Invest. 1984 Jan;73(1):1-4 PMID: 6140270
  2. Thyrotropin-releasing hormone and GTP activate inositol trisphosphate formation in membranes isolated from rat pituitary cells.
    J Biol Chem. 1986 Feb 25;261(6):2712-7 PMID: 3005261
  3. Phosphatidylinositol-4,5-bisphosphate phosphodiesterase and phosphomonoesterase activities of rat brain. Some properties and possible control mechanisms.
    Biochem J. 1984 Feb 15;218(1):177-85 PMID: 6324748
  4. Hydrolysis of polyphosphoinositides by purified sheep seminal vesicle phospholipase C enzymes.
    J Biol Chem. 1984 Oct 10;259(19):11718-24 PMID: 6090445
  5. Thyrotropin-releasing hormone activates a Ca2+-dependent polyphosphoinositide phosphodiesterase in permeable GH3 cells. GTP gamma S potentiation by a cholera and pertussis toxin-insensitive mechanism.
    J Biol Chem. 1986 Feb 25;261(6):2918-27 PMID: 3005271
  6. Phospholipase C in rat liver plasma membranes. Phosphoinositide specificity and regulation by guanine nucleotides and calcium.
    FEBS Lett. 1986 Mar 17;198(1):85-8 PMID: 3007209
  7. A guanine nucleotide-dependent phosphatidylinositol 4,5-diphosphate phospholipase C in cells transformed by the v-fms and v-fes oncogenes.
    J Biol Chem. 1986 Apr 15;261(11):4978-85 PMID: 3007485
  8. Partial purification of phosphoinositide phospholipase C from human platelet cytosol; characterization of its three forms.
    Biochem Biophys Res Commun. 1986 Apr 29;136(2):713-21 PMID: 3010993
  9. Protein kinase C activation by diacylglycerol second messengers.
    Cell. 1986 Jun 6;45(5):631-2 PMID: 3708690
  10. Studies and perspectives of protein kinase C.
    Science. 1986 Jul 18;233(4761):305-12 PMID: 3014651
  11. Studies on the hepatic calcium-mobilizing activity of aluminum fluoride and glucagon. Modulation by cAMP and phorbol myristate acetate.
    J Biol Chem. 1986 Aug 25;261(24):11056-63 PMID: 2426266
  12. Regulation of membrane-associated and cytosolic phospholipase C activities in human platelets by guanosine triphosphate.
    J Biol Chem. 1986 Sep 15;261(26):11942-4 PMID: 3017954
  13. Phosphatidylinositol-specific phospholipase C of murine lymphocytes.
    Arch Biochem Biophys. 1986 Sep;249(2):569-78 PMID: 3753018
  14. Guanine nucleotides stimulate soluble phosphoinositide-specific phospholipase C in the absence of membranes.
    J Biol Chem. 1986 Dec 15;261(35):16553-8 PMID: 3023354
  15. Regulation of the phosphoinositide hydrolysis pathway in thrombin-stimulated platelets by a pertussis toxin-sensitive guanine nucleotide-binding protein. Evaluation of its contribution to platelet activation and comparisons with the adenylate cyclase inhibitory protein, Gi.
    J Biol Chem. 1986 Dec 25;261(36):16838-47 PMID: 3023367
  16. The metabolism of phosphoinositide-derived messenger molecules.
    Science. 1986 Dec 19;234(4783):1519-26 PMID: 3024320
  17. Characterization of multiple forms of phosphoinositide-specific phospholipase C purified from human platelets.
    Biochem J. 1986 Jul 1;237(1):139-45 PMID: 3026307
  18. Guanine nucleotide regulation of phospholipase C activity in permeabilized rabbit neutrophils. Inhibition by pertussis toxin and sensitization to submicromolar calcium concentrations.
    Biochem J. 1986 Oct 1;239(1):97-102 PMID: 3541923
  19. Two forms of phosphatidylinositol-specific phospholipase C from bovine brain.
    Biochem Biophys Res Commun. 1986 Nov 26;141(1):137-44 PMID: 3541924
  20. Evidence for a GTP-binding protein coupling thrombin receptor to PIP2-phospholipase C in membranes of hamster fibroblasts.
    FEBS Lett. 1987 Jan 1;210(1):6-10 PMID: 3026838
  21. Guanosine 5'-O-(thiotriphosphate)-dependent inositol trisphosphate formation in membranes is inhibited by phorbol ester and protein kinase C.
    J Biol Chem. 1987 Feb 5;262(4):1638-43 PMID: 3543007
  22. Mechanisms involved in calcium-mobilizing agonist responses.
    Adv Cyclic Nucleotide Protein Phosphorylation Res. 1986;20:211-62 PMID: 3028085
  23. The dependence on Ca2+ of the guanine-nucleotide-activated polyphosphoinositide phosphodiesterase in neutrophil plasma membranes.
    Biochem J. 1986 Dec 1;240(2):503-7 PMID: 3028376
  24. Partial purification and characterization of membrane-bound and cytosolic phosphatidylinositol-specific phospholipases C from murine thymocytes.
    J Biochem. 1986 Oct;100(4):1015-22 PMID: 3493241
  25. Thrombin- and nucleotide-activated phosphatidylinositol 4,5-bisphosphate phospholipase C in human platelet membranes.
    J Biol Chem. 1987 Apr 25;262(12):5492-8 PMID: 3032933
  26. Purification of polyphosphoinositides by chromatography on immobilized neomycin.
    J Lipid Res. 1978 Nov;19(8):1063-7 PMID: 215685
  27. Purification of phosphatidylinositol-specific phospholipase C from rat liver.
    J Biol Chem. 1981 Jul 10;256(13):6769-75 PMID: 7240243
  28. Cooperative roles of various membrane phospholipids in the activation of calcium-activated, phospholipid-dependent protein kinase.
    J Biol Chem. 1981 Jul 25;256(14):7146-9 PMID: 7251589
  29. Modulation of phosphatidylinositol-specific phospholipase C activity by phospholipid interactions, diglycerides, and calcium ions.
    J Biol Chem. 1982 Dec 10;257(23):14359-64 PMID: 7142213
  30. Rapid accumulation of inositol trisphosphate reveals that agonists hydrolyse polyphosphoinositides instead of phosphatidylinositol.
    Biochem J. 1983 Jun 15;212(3):849-58 PMID: 6309155
  31. Inositol trisphosphate, a novel second messenger in cellular signal transduction.
    Nature. 1984 Nov 22-28;312(5992):315-21 PMID: 6095092
  32. 5-Hydroxytryptamine stimulates inositol phosphate production in a cell-free system from blowfly salivary glands. Evidence for a role of GTP in coupling receptor activation to phosphoinositide breakdown.
    J Biol Chem. 1985 May 10;260(9):5464-71 PMID: 2985595
  33. Role of guanine nucleotide binding protein in the activation of polyphosphoinositide phosphodiesterase.
    Nature. 1985 Apr 11-17;314(6011):534-6 PMID: 2986003
  34. Chemoattractant receptor-induced hydrolysis of phosphatidylinositol 4,5-bisphosphate in human polymorphonuclear leukocyte membranes. Requirement for a guanine nucleotide regulatory protein.
    J Biol Chem. 1985 May 25;260(10):5875-8 PMID: 2987206
  35. Guanosine 5'-O-thiotriphosphate stimulates phospholipase C activity in plasma membranes of rat hepatocytes.
    J Biol Chem. 1985 Aug 15;260(17):9527-30 PMID: 2991250
  36. The relationships between receptor binding capacity for norepinephrine, angiotensin II, and vasopressin and release of inositol trisphosphate, Ca2+ mobilization, and phosphorylase activation in rat liver.
    Mol Pharmacol. 1985 Aug;28(2):93-9 PMID: 2991741
  37. Rat brain and liver soluble phospholipase C: resolution of two forms with different requirements for calcium.
    Biochem Biophys Res Commun. 1985 Oct 30;132(2):582-90 PMID: 2998375
  38. 5-Methyltryptamine stimulates phospholipase C-mediated breakdown of exogenous phosphoinositides by blowfly salivary gland membranes.
    J Biol Chem. 1985 Dec 25;260(30):16052-5 PMID: 2999146
  39. Dependence on Ca2+ of the activities of phosphatidylinositol 4,5-bisphosphate phosphodiesterase and inositol 1,4,5-trisphosphate phosphatase in smooth muscles of the porcine coronary artery.
    Biochem J. 1985 Nov 1;231(3):497-503 PMID: 3000351
  40. Hormone-stimulated polyphosphoinositide breakdown in rat liver plasma membranes. Roles of guanine nucleotides and calcium.
    J Biol Chem. 1986 Feb 15;261(5):2140-6 PMID: 3003097
  41. Guanine nucleotides stimulate production of inositol trisphosphate in rat cortical membranes.
    Biochem J. 1985 Dec 15;232(3):799-804 PMID: 3004420
  42. Vasopressin-, angiotensin II-, and alpha 1-adrenergic-induced inhibition of Ca2+ transport by rat liver plasma membrane vesicles.
    J Biol Chem. 1984 Feb 10;259(3):1382-5 PMID: 6693408
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2480791
Published
1987-12-15
Pages
791-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148619
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2480791 PMC Full Text View journal details
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