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PMID: 2830591 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Human transposon-like elements insert at a preferred target site: evidence for a retrovirally mediated process.

Nucleic acids research ·Vol. 16 ·No. 3 ·1988-02-11 ·Pages 1143-51

Deka N, Willard CR, Wong E, Schmid CW

Abstract

Members of the human transposon-like family of repetitive sequences (called THE 1 repeats) like many other repetitive DNA sequences are flanked by short direct repeats. Comparison of the base sequences of twelve examples of these flanking direct repeats indicates that THE 1 repeats insert into a preferred genomic target site. In one case, we have identified the sequence of an empty site into which a THE 1 element inserted. The sequence of this empty site and sequences of truncated THE 1 LTRs are consistent with a retroviral mechanism for the insertion of THE 1 elements. Truncated transposon structures illustrate for the first time that intermediate structures of retrotransposition may also be integrated into the genome.

MeSH Terms
Base Sequence DNA Transposable Elements DNA, Recombinant Humans Proviruses Recombination, Genetic Repetitive Sequences, Nucleic Acid Retroviridae/physiology Sequence Homology, Nucleic Acid
Chemicals
DNA Transposable Elements DNA, Recombinant
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Deka N
Department of Chemistry, University of California, Davis 95616.
Willard C R
Wong E
Schmid C W
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.3.1143
Published
1988-02-11
Pages
1143-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334743
Subset
IM
Grants
NIGMS NIH HHS · GM 21346 · United States
External Links
PubMed source DOI: 10.1093/nar/16.3.1143 PMC Full Text View journal details
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