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PMID: 2995920 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Site-specific nicking at the avian retrovirus LTR circle junction by the viral pp32 DNA endonuclease.

Nucleic acids research ·Vol. 13 ·No. 17 ·1985-09-11 ·Pages 6205-21

Grandgenett DP, Vora AC

Abstract

The avian retrovirus pp32 DNA endonuclease prefers to nick supercoiled DNA containing long terminal repeat (LTR) circle junction sequences at one or the other of two sites, each which mapped two nucleotides back from the circle junction. The sequence at the sites of nicking was (sequence: see text) where increases indicates the positions of the two alternative nicked sites. This site-specific nicking was observed when the circle junction LTR DNA was present in supercoiled form, the divalent metal ion was Mg2+ and the molar ratio of protein to DNA was low. The majority of other LTR DNA sites nicked by pp32 in the presence of Mg2+ were adjacent to or within the dinucleotide CA.

MeSH Terms
Avian Sarcoma Viruses/enzymology,genetics Base Sequence DNA Restriction Enzymes Endodeoxyribonucleases/genetics Genes Genes, Viral Plasmids Repetitive Sequences, Nucleic Acid Retroviridae Proteins/genetics
Chemicals
Retroviridae Proteins avian retrovirus proteins Endodeoxyribonucleases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Grandgenett D P
Vora A C
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29 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.17.6205
Published
1985-09-11
Pages
6205-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321947
Subset
IM
Grants
NCI NIH HHS · CA-16312 · United States
External Links
PubMed source DOI: 10.1093/nar/13.17.6205 PMC Full Text View journal details
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