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PMID: 2832383 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Effects of oxygen stress on membrane functions in Escherichia coli: role of HPI catalase.

Journal of bacteriology ·Vol. 170 ·No. 4 ·1988-04-00 ·Pages 1837-42

Farr SB, Touati D, Kogoma T

Abstract

Different conditions of oxidative stress were used to study their effects on membrane transport in Escherichia coli K-12. The oxidizing conditions included H2O2, plumbagin (a redox cycling compound that generates superoxide radicals [O2-]), and increased partial pressure of oxygen. Both superoxide radical-generating conditions and H2O2 treatments were found to cause a rapid decrease in proton motive force-dependent and -independent transport. H2O2-pretreated cells had the ability to rapidly recover both proton motive force-dependent and -independent transport. The induction required transcription and translation and was dependent on oxyR+ and katG+, providing evidence that these genes play crucial roles in the rapid recovery of transport. The effects of oxidatively induced loss of proton motive force on cell growth and macromolecular synthesis were also investigated.

MeSH Terms
Biological Transport, Active Catalase/genetics,metabolism Cell Membrane/metabolism Chloramphenicol/pharmacology Escherichia coli/drug effects,genetics,metabolism,ultrastructure Genes, Bacterial Genes, Regulator Hydrogen Peroxide/pharmacology Mutation Naphthoquinones/pharmacology Oxidation-Reduction Oxygen/metabolism,pharmacology Protein Biosynthesis Protons Rifampin/pharmacology Transcription, Genetic
Chemicals
Naphthoquinones Protons Chloramphenicol Hydrogen Peroxide Catalase Oxygen Rifampin plumbagin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Farr S B
Department of Cell Biology, School of Medicine, University of New Mexico, Albuquerque.
Touati D
Kogoma T
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21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.4.1837-1842.1988
Published
1988-04-00
Pages
1837-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211039
Subset
IM
Grants
NIGMS NIH HHS · GM 22092 · United States
External Links
PubMed source DOI: 10.1128/jb.170.4.1837-1842.1988 PMC Full Text View journal details
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