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PMID: 2836702 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A point mutation in the Nul gene of bacteriophage lambda facilitates phage growth in Escherichia coli with himA and gyrB mutations.

Molecular & general genetics : MGG ·Vol. 212 ·No. 1 ·1988-04-00 ·Pages 149-56

Granston AE, Alessi DM, Eades LJ, Friedman DI

Abstract

A mutant of lambda was isolated that grows in the Escherichia coli himA delta/gyrB-him320(Ts) double mutant at 42 degrees C; conditions which are non-permissive for wild-type lambda growth. The responsible mutation, ohm1, alters the 40th codon of the Nul reading frame. The Nul and A gene products comprise the terminase protein which cleaves concatameric DNA into unit-length phage genomes during DNA packaging. The Nul-ohm1 gene product acts in trans to support lambda growth in the double himA/gyrB mutant, and lambda cos154 growth in the single himA mutant. The observation that an alteration in Nul suppresses the inhibition of growth in the double himA/gyrB mutant implicates DNA gyrase, as well as integration host factor, in the DNA:protein interactions that occur at the initiation of packaging.

MeSH Terms
Bacteriophage lambda/enzymology,genetics,growth & development DNA Topoisomerases, Type II/genetics Endodeoxyribonucleases/genetics Escherichia coli/genetics Genes Genes, Viral Genetic Complementation Test Mutation
Chemicals
Endodeoxyribonucleases terminase DNA Topoisomerases, Type II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Granston A E
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109.
Alessi D M
Eades L J
Friedman D I
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00322458
Published
1988-04-00
Pages
149-56
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · 5 RO1 AI14363-11 · United States
NIGMS NIH HHS · 5-T32-GM07315-13 · United States
External Links
PubMed source DOI: 10.1007/BF00322458
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