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PMID: 2967421 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Bacteriophage lambda DNA packaging: a mutant terminase that is independent of integration host factor.

Molecular & general genetics : MGG ·Vol. 212 ·No. 1 ·1988-04-00 ·Pages 142-8

Feiss M, Fogarty S, Christiansen S

Abstract

Lambda+ is able to grow in Escherichia coli cells lacking integration host factor (IHF), producing a burst of approximately 25% that produced in IHF+ cells. In vitro, however, we find that the lambda DNA packaging enzyme terminase is strongly dependent on IHF in both cos cleavage reactions and DNA packaging reactions. The cos59 mutation renders lambda dependent on IHF in vivo. The cos59 mutation is a deletion of 3 base pairs at the XmnI site in the cohesive end site (cos) of lambda. Variants of lambda cos59 that were able to grow in the absence of IHF were isolated and found to carry a mutation, called ms1, in the Nu1 gene, which codes for the small subunit of terminase. The Nu1ms1 mutation results in a change of the 40th amino acid of the Nu1 gene product from leucine to phenylalanine. The Nu1ms1 terminase was independent of IHF in packaging reactions in vitro. The results indicate that the mutation either renders terminase: (1) able to utilize some host protein other than IHF, or (2) totally independent of host factors.

MeSH Terms
Bacterial Proteins/genetics Bacteriophage lambda/enzymology,genetics DNA, Viral/genetics DNA-Binding Proteins/genetics Endodeoxyribonucleases/genetics Escherichia coli/genetics Genes Genes, Viral Integration Host Factors Mutation
Chemicals
Bacterial Proteins DNA, Viral DNA-Binding Proteins Integration Host Factors Endodeoxyribonucleases terminase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Feiss M
Department of Microbiology, College of Medicine, University of Iowa, Iowa City.
Fogarty S
Christiansen S
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00322457
Published
1988-04-00
Pages
142-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIAID NIH HHS · AI-12851 · United States
External Links
PubMed source DOI: 10.1007/BF00322457
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