A DNA sequence outside the pUB110 minimal replicon is required for normal replication in Bacillus subtilis.

Nucleic acids research ·Vol. 16 ·No. 10 ·1988-05-25 ·Pages 4389-406

Viret JF, Alonso JC

Abstract

The origin of lagging strand synthesis in pUB110, oriL, has been localized within 140 bases outside the pUB110 minimal replicon. The oriL DNA sequence is a cis-acting and orientation dependent determinant required for normal plasmid replication. Rearrangements affecting oriL cause plasmid instability, lead to the accumulation of replication intermediates and result in a marked reduction of the plasmid copy number in some recombination deficient mutant strains. In addition, deletion of oriL triggers a dnaB-dependent mode of replication. Insertion of the functionally asymmetric oriL region in the proper orientation into pC194 reduces the accumulation of single-stranded DNA during the replication of this plasmid.

MeSH Terms

Bacillus subtilis/genetics DNA Replication DNA Restriction Enzymes Plasmids Replicon Transduction, Genetic Transformation, Bacterial

Chemicals

DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Viret J F

Max-Planck-Institut für Molekulare Genetik, Berlin, FRG.

Alonso J C

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25 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.10.4389

Published

1988-05-25

Pages

4389-406

Language

English

Region

England

NLM ID

0411011

PMCID

PMC336637

Subset

External Links

PubMed source DOI: 10.1093/nar/16.10.4389 PMC Full Text View journal details

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A DNA sequence outside the pUB110 minimal replicon is required for normal replication in Bacillus subtilis.

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