Isolation and characterization of the minimal fragment required for autonomous replication ("basic replicon") of a copy mutant (pKN102) of the antibiotic resistance factor R1.

Molecular & general genetics : MGG ·Vol. 162 ·No. 1 ·1978-06-01 ·Pages 51-7

Kollek R, Oertel W, Goebel W

Abstract

The mini plasmids deriving from pKN102, a copy mutant of the antibiotic resistance factor R1drd-19 of E. coli, share a common DNA sequence of 2.6 kb, which carries the minimal functions for autonomous replication. By cloning of two PstI fragments of this region it could be demonstrated that the "basic replicon" is a DNA segment not larger than 1.8 kb, which carries the orgin of replication and the genetic information for at least two proteins. Protein F (NW=11.000 dalton) seems to be synthesed in larger amounts in minicells of E. coli than protein C (20.000). Plasmids containing this isolated replicon of R1 are completely compatible with the parental plasmid R1drd-19.

MeSH Terms

Anti-Bacterial Agents/pharmacology Bacterial Proteins/genetics DNA Replication DNA, Bacterial/genetics Escherichia coli Mutation R Factors Transformation, Bacterial

Chemicals

Anti-Bacterial Agents Bacterial Proteins DNA, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kollek R

Oertel W

Goebel W

References (16)

16 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00333850

Published

1978-06-01

Pages

51-7

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00333850

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Isolation and characterization of the minimal fragment required for autonomous replication ("basic replicon") of a copy mutant (pKN102) of the antibiotic resistance factor R1.

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