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PMID: 330504 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Origin and direction of replication of the drug resistance plasmid R100.1 and of a resistance transfer factor derivative in synchronized cultures.

Journal of bacteriology ·Vol. 131 ·No. 3 ·1977-09-00 ·Pages 929-42

Silver L, Chandler M, de la Tour EB, Caro L

Abstract

The origin and direction of replication of the resistance plasmid R100.1 and its resistance transfer factor derivative, pAR132, were studied by electron microscopy autoradiography of partially denatured molecules and partial denaturation mapping of replicative intermediates. Results of these studies indicate the existence of an origin of replication at 8.8 kilobases on the R100 map. Replication from this origin in cultures synchronized for initiation of replication is predominantly unidirectional in a single direction.

MeSH Terms
Amino Acids/metabolism Autoradiography/methods Chromosome Mapping Conjugation, Genetic DNA Replication DNA, Bacterial/biosynthesis Drug Resistance, Microbial Escherichia coli/metabolism Nucleic Acid Denaturation R Factors Thymine/metabolism
Chemicals
Amino Acids DNA, Bacterial Thymine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Silver L
Chandler M
de la Tour E B
Caro L
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.131.3.929-942.1977
Published
1977-09-00
Pages
929-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235551
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.131.3.929-942.1977 PMC Full Text View journal details
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