Two replication initiation sites on R-plasmid DNA.
Replicating DNA molecules of a deletion mutant of the conjugative R-plasmid R 6 K are cleaved at a single site by the EcoRI restriction endonuclease. Electron microscope examination and measurements of the EcoRI treated replicative intermediate molecules indicate that replication can be initiated at two sites on the plasmid DNA molecule. The two sites are located at about 23 and 39% of total length, respectively, from the EcoRI cleavage site. About 5% of the replicating molecules use both replication initiation sites simultaneously.
17 references, click to expand
-
Unidirectional replication of plasmid ColE1 DNA.Nature. 1974 Sep 27;251(5473):337-40 PMID: 4610399
-
Origin and direction of simian virus 40 deoxyribonucleic acid replication.J Virol. 1972 Sep;10(3):484-91 PMID: 4342055
-
Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
-
Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control.J Bacteriol. 1970 Oct;104(1):34-44 PMID: 4919751
-
On the mechanism of DNA replication in mammalian chromosomes.J Mol Biol. 1968 Mar 14;32(2):327-41 PMID: 5689363
-
Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes.J Bacteriol. 1973 Sep;115(3):904-11 PMID: 4728274
-
An inocula replicating apparatus for routine testing of bacterial susceptibility to antibiotics.Antibiot Chemother (Northfield). 1959 May;9(5):307-11 PMID: 24545214
-
Replication of colicin E1 plasmid DNA in cell extracts. Origin and direction of replication.Proc Natl Acad Sci U S A. 1974 Jun;71(6):2260-4 PMID: 4601821
-
Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K.Biochemistry. 1974 Dec 31;13(27):5484-90 PMID: 4616719
-
Mechanism of DNA replication in Drosophila chromosomes: structure of replication forks and evidence for bidirectionality.Proc Natl Acad Sci U S A. 1974 Jan;71(1):135-9 PMID: 4204203
-
R6K plasmid replication: influence of chromosomal genotype in minicell-producing strains of Escherichia coli K-12.J Bacteriol. 1974 Dec;120(3):1387-400 PMID: 4612021
-
Origin of the TEM-beta-lactamase gene found on plasmids.J Bacteriol. 1975 Apr;122(1):250-6 PMID: 1091625
-
Replication of yeast chromosomal DNA.Nature. 1974 Jan 4;247(5435):32-5 PMID: 4587640
-
A catenated DNA molecule as an intermediate in the replication of the resistance transfer factor R6K in Escherichia coli.Biochem Biophys Res Commun. 1973 Oct 15;54(4):1451-9 PMID: 4585292
-
Replication of colicin E1 plasmid DNA in minicells from a unique replication initiation site.Proc Natl Acad Sci U S A. 1974 Jun;71(6):2256-9 PMID: 4601583
-
Nature of R-factor replication in the presence of chloramphenicol.Proc Natl Acad Sci U S A. 1975 Feb;72(2):654-8 PMID: 1091928
-
Characterization of the replicative intermediates of polyoma virus.Virology. 1974 Jan;57(1):147-60 PMID: 4131952
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
