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PMID: 2839522 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Recycling glycoproteins do not return to the cis-Golgi.

The Journal of cell biology ·Vol. 107 ·No. 1 ·1988-07-00 ·Pages 79-87

Neefjes JJ, Verkerk JM, Broxterman HJ, van der Marel GA, van Boom JH, Ploegh HL

Abstract

Recycling of a number of glycoproteins along the site of action of mannosidase I (the distal endoplasmic reticulum/cis-Golgi) was followed in several different cell lines. Treatment of cells with 1-deoxymannojirimycin (dMM) produced high mannose oligosaccharides at positions otherwise occupied by complex-type oligosaccharides in these glycoproteins. Conversion of high-mannose-type oligosaccharides to complex oligosaccharides of proteins initially synthesized in the presence of dMM was used as a marker for recycling of glycoproteins along the site of action of dMM. In contrast to findings reported by Snider and Rogers (Snider, M. D., and O. C. Rogers. 1986. J. Cell Biol. 103:265-275), removal of dMM did not result in reconversion of high-mannose oligosaccharides to complex-type sugars, even after prolonged periods of culture. We conclude that surface glycoproteins do not recycle through the cis-medial Golgi elements.

MeSH Terms
1-Deoxynojirimycin Anti-Bacterial Agents/pharmacology Carcinoma, Hepatocellular Cell Line, Transformed Electrophoresis, Polyacrylamide Gel Endoplasmic Reticulum/enzymology,metabolism Glucosamine/analogs & derivatives,pharmacology Glycoproteins/metabolism Golgi Apparatus/enzymology,metabolism HLA Antigens HLA-D Antigens Humans Immunoassay Isoelectric Focusing Leukemia, Erythroblastic, Acute Liver Neoplasms Mannosidases/antagonists & inhibitors Neuraminidase/metabolism Receptors, Transferrin/drug effects,metabolism Transferrin/metabolism Tumor Cells, Cultured
Chemicals
Anti-Bacterial Agents Glycoproteins HLA Antigens HLA-D Antigens Receptors, Transferrin Transferrin 1-Deoxynojirimycin Mannosidases mannosyl-oligosaccharide 1,2-alpha-mannosidase Neuraminidase Glucosamine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Neefjes J J
Netherlands Cancer Institute, Amsterdam.
Verkerk J M
Broxterman H J
van der Marel G A
van Boom J H
Ploegh H L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.107.1.79
Published
1988-07-00
Pages
79-87
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115183
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.107.1.79 PMC Full Text View journal details
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