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PMID: 2842314 Published · ppublish English Journal Article

Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination.

Journal of bacteriology ·Vol. 170 ·No. 9 ·1988-09-00 ·Pages 4322-9

Shinagawa H, Makino K, Amemura M, Kimura S, Iwasaki H, Nakata A

Abstract

The ruv gene of Escherichia coli, which is involved in DNA repair and recombination, was cloned on a plasmid vector. The DNA of the ruv region was sequenced; it had two open reading frames in tandem that could code for 22- and 37-kilodalton proteins. The proteins encoded by these open reading frames were identified by the maxicell method. The two genes were aligned in the same orientation and regulated by the SOS system, so the two genes probably constitute an operon. The distal one complemented the ruv mutations. Transcription of the operon was studied both in vivo and in vitro. Two transcription initiation sites were identified upstream of the coding frames, and the transcription from both sites was repressed by the LexA repressor. A DNA sequence that is homologous to the SOS box and bound by LexA protein was found in the regulatory region of the operon. The amino acid sequence of Ruv protein deduced from the DNA sequence shows a high degree of homology to the consensus sequence shared by ATP-binding proteins.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Base Sequence Cloning, Molecular DNA Repair DNA Restriction Enzymes DNA, Bacterial/genetics Endonucleases Escherichia coli/genetics Gene Expression Regulation Genes, Bacterial Genetic Vectors Molecular Sequence Data Operon Plasmids Protein Biosynthesis Recombination, Genetic Repressor Proteins/metabolism SOS Response, Genetics Serine Endopeptidases Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial LexA protein, Bacteria Repressor Proteins Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases Serine Endopeptidases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Shinagawa H
Department of Experimental Chemotherapy, Osaka University, Japan.
Makino K
Amemura M
Kimura S
Iwasaki H
Nakata A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.9.4322-4329.1988
Published
1988-09-00
Pages
4322-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211445
Subset
IM
Databases
GENBANK
M21298
External Links
PubMed source DOI: 10.1128/jb.170.9.4322-4329.1988 PMC Full Text View journal details
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