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PMID: 2989817 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural analysis of the umu operon required for inducible mutagenesis in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 13 ·1985-07-00 ·Pages 4336-40

Kitagawa Y, Akaboshi E, Shinagawa H, Horii T, Ogawa H, Kato T

Abstract

The nucleotide sequence of a 2.8-kilobase fragment of the Escherichia coli chromosome containing the umuDC genes has been determined. The DNA sequence specifies two open reading frames of 417 and 1266 nucleotides encoding proteins with calculated molecular weights of 15,063 and 47,677, respectively. From these and the previous results of genetic and biochemical studies on the cloned genes, we conclude that the former is the umuD and the latter is the umuC gene. In vitro transcription of the regulatory region of the umu operon revealed that (i) upstream of the coding region there is a promoter-operator complex having the consensus sequence, CTGTATATAAAAACAG, of an SOS box, (ii) transcription of the umu operon begins at an adenine residue in the SOS box, and (iii) the LexA protein binds to the operator region, with an equilibrium dissociation constant (Kd) of 0.2, thereby repressing the transcription of the umuDC genes. These results indicate that the expression of the umu operon is under the coordinated control of the recA lexA gene products.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/analysis,genetics,metabolism Base Sequence Binding Sites DNA Repair DNA Restriction Enzymes/metabolism DNA, Bacterial/analysis Deoxyribonuclease I/metabolism Endoribonucleases/metabolism Escherichia coli/genetics Gene Expression Regulation Mutation Operon Rec A Recombinases/metabolism Ribonuclease T1/metabolism Ribonuclease, Pancreatic/metabolism Serine Endopeptidases
Chemicals
Bacterial Proteins DNA, Bacterial LexA protein, Bacteria Rec A Recombinases Endoribonucleases DNA Restriction Enzymes Deoxyribonuclease I ribonuclease U Ribonuclease T1 Ribonuclease, Pancreatic Serine Endopeptidases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kitagawa Y
Akaboshi E
Shinagawa H
Horii T
Ogawa H
Kato T
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.13.4336
Published
1985-07-00
Pages
4336-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390408
Subset
IM
Databases
GENBANK
M10107
External Links
PubMed source DOI: 10.1073/pnas.82.13.4336 PMC Full Text View journal details
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