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PMID: 28426965 Published · ppublish English Journal Article

Formation and Maintenance of Functional Spines in the Absence of Presynaptic Glutamate Release.

Neuron ·Vol. 94 ·No. 2 ·2017-04-19 ·Pages 304-311.e4

Sigler A, Oh WC, Imig C, Altas B, Kawabe H, Cooper BH, Kwon HB, Rhee JS, Brose N

Abstract

Dendritic spines are the major transmitter reception compartments of glutamatergic synapses in most principal neurons of the mammalian brain and play a key role in the function of nerve cell circuits. The formation of functional spine synapses is thought to be critically dependent on presynaptic glutamatergic signaling. By analyzing CA1 pyramidal neurons in mutant hippocampal slice cultures that are essentially devoid of presynaptic transmitter release, we demonstrate that the formation and maintenance of dendrites and functional spines are independent of synaptic glutamate release.

Keywords
activity dependence dendrite formation glutamate release glutamate uncaging spine formation synaptogenesis
MeSH Terms
Animals Calcium/metabolism Dendrites/metabolism Dendritic Spines/metabolism Glutamic Acid/metabolism Hippocampus/metabolism Mice Signal Transduction/physiology Synapses/metabolism,physiology
Chemicals
Glutamic Acid Calcium
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Sigler Albrecht
Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany; Science Products GmbH, 65719 Hofheim am Taunus, Germany.
Oh Won Chan
Research Group Cellular Basis of Neural Circuit Plasticity, Max Planck Florida Institute for Neuroscience, Jupiter, FL 33458, USA.
Imig Cordelia
Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.
Altas Bekir
Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.
Kawabe Hiroshi
Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.
Cooper Benjamin H
Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany.
Kwon Hyung-Bae
Research Group Cellular Basis of Neural Circuit Plasticity, Max Planck Florida Institute for Neuroscience, Jupiter, FL 33458, USA; Max Planck Institute of Neurobiology, 82152 Martinsried, Germany.
Rhee Jeong-Seop
Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany. Electronic address: [email protected].
Brose Nils
Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075 Göttingen, Germany. Electronic address: [email protected].
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
1097-4199
DOI
10.1016/j.neuron.2017.03.029
Email
[email protected]
[email protected]
Published
2017-04-19
Pages
304-311.e4
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC5418202
Subset
IM
Grants
NIMH NIH HHS · R01 MH107460 · United States
External Links
PubMed source DOI: 10.1016/j.neuron.2017.03.029 PMC Full Text View journal details
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