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PMID: 2842791 Published · ppublish English Journal Article

Antisense Myc sequences induce differentiation of F9 cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 18 ·1988-09-00 ·Pages 6806-10

Griep AE, Westphal H

Abstract

Down-regulation of Myc expression is the earliest documented change in gene expression in retinoic acid-induced differentiation of murine F9 teratocarcinoma cells. F9 cells transfected with plasmids expressing antisense Myc sequences under control of the simian virus 40 (SV40) early promoter exhibit a decrease in Myc protein. The result of this decrease is the spontaneous differentiation into cells that resemble retinoic acid-treated F9 cells as judged by plasminogen activator assays. In contrast, when F9 cells are transfected with a plasmid expressing Myc under control of the SV40 early promoter, resulting cell clones are resistant to differentiation by retinoic acid as shown by the lack of induction of plasminogen activator. These results suggest that down-regulation of Myc is sufficient and necessary for F9 cell differentiation.

MeSH Terms
Animals Cell Differentiation Cell Line Oncogenes Phenotype Plasmids Simian virus 40/genetics Teratoma/genetics Transfection Tretinoin/pharmacology
Chemicals
Tretinoin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Griep A E
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, MD 20892.
Westphal H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.18.6806
Published
1988-09-00
Pages
6806-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282067
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.18.6806 PMC Full Text View journal details
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