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PMID: 2825168 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cell-specific activity of the constituent elements of the simian virus 40 enhancer.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 22 ·1987-11-00 ·Pages 7881-5

Nomiyama H, Fromental C, Xiao JH, Chambon P

Abstract

We have compared the activation of transcription from the early promoter of simian virus 40 brought about by the enhancer during transient expression in several cell lines. The enhancer stimulated RNA synthesis approximately 400-, 100-, 100-, and 300-fold in human HeLa cells, the mouse plasmacytoma B cells MPC11, and the nondifferentiated and retinoic acid-differentiated mouse embryonal carcinoma F9 cells, respectively. The contribution to enhancer activity of its multiple constituent motifs has been investigated in the different cell types using the pA series of recombinants mutated throughout the enhancer. A comparison of the activity of these recombinants in the four cell lines indicates that the activity of the various enhancer motifs is cell specific. The activity of a given motif in a given cell line correlates well with the known presence in this cell line of a protein(s) that binds specifically to that motif in vitro.

MeSH Terms
Animals Cell Line Enhancer Elements, Genetic Genes, Viral HeLa Cells/metabolism Humans Mice Plasmacytoma/pathology Simian virus 40/genetics Teratoma/pathology Transcription Factors/metabolism Tumor Cells, Cultured/metabolism
Chemicals
Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nomiyama H
Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Strasbourg, France.
Fromental C
Xiao J H
Chambon P
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.22.7881
Published
1987-11-00
Pages
7881-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299438
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.22.7881 PMC Full Text View journal details
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