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PMID: 3035489 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Competition studies with repressors and activators of viral enhancer function in F9 mouse embryonal carcinoma cells.

Nucleic acids research ·Vol. 15 ·No. 10 ·1987-05-26 ·Pages 4307-24

Sleigh MJ, Lockett TJ, Kelly J, Lewy D

Abstract

DNA competition studies have been used to investigate the presence of a repressor of viral enhancer function in F9 mouse embryonal carcinoma cells. The complete polyoma virus enhancer region, cotransfected into F9 cells with the SV40 promoter/enhancer attached to a chloramphenicol acetyl transferase marker gene, induced a small increase in pSV2CAT expression. This can be explained by preferential but weak binding by polyoma sequences of a molecule repressing pSV2CAT transcription. Repressor activity substantially disappeared when the cells were induced to differentiate by retinoic acid. Repressor binding was localised to one half of the polyoma enhancer, but was lost on further fragmentation of this region. It appears that multiple sequence elements may be required for repressor binding and that these are at least partially separable from the complement of elements binding enhancer activating molecules.

MeSH Terms
Animals Cell Differentiation/drug effects Cell Line Cyclic AMP/physiology DNA, Bacterial/genetics,pharmacology DNA, Recombinant/pharmacology Enhancer Elements, Genetic/drug effects Gene Expression Regulation/drug effects Genes, Regulator/drug effects Genes, Synthetic Genes, Viral/drug effects Mice Polyomavirus/genetics Promoter Regions, Genetic Recombinant Fusion Proteins/biosynthesis,genetics Repressor Proteins/metabolism Simian virus 40/genetics Teratoma/genetics,pathology Transcription Factors/metabolism Transfection Tretinoin/pharmacology
Chemicals
DNA, Bacterial DNA, Recombinant Recombinant Fusion Proteins Repressor Proteins Transcription Factors Tretinoin Cyclic AMP
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sleigh M J
Lockett T J
Kelly J
Lewy D
References (37)
37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.10.4307
Published
1987-05-26
Pages
4307-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340849
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.10.4307 PMC Full Text View journal details
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