Home LiteratureArticle Details
PMID: 284326 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Lateral diffusion in binary mixtures of cholesterol and phosphatidylcholines.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 1 ·1979-01-00 ·Pages 15-8

Rubenstein JL, Smith BA, McConnell HM

Abstract

The lateral diffusion of a fluorescent-labeled phospholipid, phosphatidyl-N-(4-nitrobenzo-2-oxa-1,3,-diazole)ethanolamine, has been measured in binary mixtures of cholesterol and dimyristoyl phosphatidylcholine at temperatures both above and below 23.8 degrees C, the chain-melting transition temperature of this phosphatidylcholine. There is a temperature-composition region, approximately temperature less than 23 degrees C and mole fraction of cholesterol (X) less than 0.20, in which the lateral diffusion coefficient of the fluorescent probe is at least an order of magnitude smaller than it is at points outside of this temperature-composition region. At temperatures above approximately 23 degrees C there is a significant increasing cholesterol concentration, for X greater than 0.2.

MeSH Terms
Cholesterol Diffusion Membrane Fluidity Membranes, Artificial Models, Biological Phosphatidylcholines Temperature
Chemicals
Membranes, Artificial Phosphatidylcholines Cholesterol
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rubenstein J L
Smith B A
McConnell H M
References (26)
26 references, click to expand
  1. A deuterium nuclear magnetic resonance study of the condensing effect of cholesterol on egg phosphatidylcholine bilayer membranes. I. Perdeuterated fatty acid probes.
    Chem Phys Lipids. 1976 Oct;17(2-3 SPEC NO):251-63 PMID: 1033045
  2. Dynamics of lipids in membranes: Heterogeneity and the role of cholesterol.
    FEBS Lett. 1972 Jul 1;23(3):285-297 PMID: 11946637
  3. The distribution of cholesterol in bilayers of phosphatidylcholines as visualized by freeze fracturing.
    Biochim Biophys Acta. 1974 Dec 24;373(3):495-501 PMID: 4433591
  4. Spectroscopic studies of specifically deuterium labeled membrane systems. Nuclear magnetic resonance investigation of the effects of cholesterol in model systems.
    Biochemistry. 1978 Jul 11;17(14):2727-40 PMID: 687560
  5. Molecular motion and order in oriented lipid multibilayer membranes evaluated by simulations of spin label ESR spectra. Effects of temperature, cholesterol and magnetic field.
    Biochim Biophys Acta. 1978 Apr 4;508(2):213-35 PMID: 205243
  6. Clustering of nitroxide spin labels in lipid bilayer membranes.
    J Am Chem Soc. 1977 Mar 2;99(5):1637-42 PMID: 839011
  7. Structural and dynamical aspects of membrane immunochemistry using model membranes.
    Biochemistry. 1977 Mar 22;16(6):1209-17 PMID: 191064
  8. Multiple phase equilibria in binary mixtures of phospholipids.
    Biochim Biophys Acta. 1978 Jun 2;509(3):462-73 PMID: 580767
  9. Equilibrium studies of lecithin-cholesterol interactions I. Stoichiometry of lecithin-cholesterol complexes in bulk systems.
    Biophys J. 1978 Jun;22(3):469-88 PMID: 667296
  10. Relation of lateral molecular motion in membranes and immune response.
    Harvey Lect. 1978;72:231-52 PMID: 750550
  11. The preferential interaction of cholesterol with different classes of phospholipids.
    Biochim Biophys Acta. 1977 Feb 14;465(1):1-10 PMID: 836830
  12. Model lipid bilayer membranes as tragets for antibody-dependent, cellular- and complement-mediated immune attack.
    Ann N Y Acad Sci. 1978;308:124-38 PMID: 279285
  13. Investigation of phase transitions of lipids and lipid mixtures by sensitivity differential scanning calorimetry.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3862-6 PMID: 1069270
  14. The effect of cholesterol incorporation on the temperature dependence of water permeation through liposomal membranes prepared from phosphatidylcholines.
    Biochim Biophys Acta. 1977 Feb 4;464(3):509-18 PMID: 836824
  15. Antigen mobility in membranes and complement-medical immune attack.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2483-7 PMID: 1058468
  16. The effect of the polar headgroup on the lipid-cholesterol interaction: a monolayer and differential scanning calorimetry study.
    Biochim Biophys Acta. 1973 Apr 25;307(1):1-19 PMID: 4711186
  17. Studies on the anomalous thermotropic behavior of aqueous dispersions of dipalmitoylphosphatidylcholine-cholesterol mixtures.
    Biochemistry. 1978 May 16;17(10):1984-9 PMID: 580764
  18. Determination of molecular motion in membranes using periodic pattern photobleaching.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2759-63 PMID: 275845
  19. Reconstitution of band 3, the erythrocyte anion exchange protein.
    Biochem Biophys Res Commun. 1977 Feb 21;74(4):1318-25 PMID: 843364
  20. Lateral phase separations in membranes.
    J Supramol Struct. 1973;1(4):285-94 PMID: 4588281
  21. An accurate and convenient organic phosphorus assay.
    Anal Biochem. 1971 Feb;39(2):527-30 PMID: 4324534
  22. Lateral phase separations in binary mixtures of cholesterol and phospholipids.
    Biochem Biophys Res Commun. 1973 Jul 17;53(2):446-51 PMID: 4352068
  23. High-sensitivity scanning calorimetric study of mixtures of cholesterol with dimyristoyl- and dipalmitoylphosphatidylcholines.
    Biochemistry. 1978 Jun 13;17(12):2464-8 PMID: 581060
  24. An interacting spin label study of the fluidizing and condensing effects of cholesterol on lecithin bilayers.
    Biochim Biophys Acta. 1973 Mar 16;298(2):133-44 PMID: 4352487
  25. Influence of cholesterol on the polar region of phosphatidylcholine and phosphatidylethanolamine bilayers.
    Biochemistry. 1978 Jan 24;17(2):381-4 PMID: 619997
  26. Interactions of proteins and cholesterol with lipids in bilayer membranes.
    Biochim Biophys Acta. 1976 Jan 21;419(2):206-22 PMID: 174727
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.1.15
Published
1979-01-00
Pages
15-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC382866
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.1.15 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2