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PMID: 2843395 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intracellular analysis of potentiation of CA1 hippocampal synaptic transmission by phorbol ester application.

Experimental brain research ·Vol. 71 ·No. 3 ·1988-00-00 ·Pages 588-96

Hvalby O, Reymann K, Andersen P

Abstract

(1) The effect of active and inactive phorbol esters on synaptic transmission and on membrane properties of CA1 pyramidal cells in hippocampus have been analyzed by intracellular recording. (2) 4 beta-phorbol-12,13 dibutyrate (beta PDBu), but not the alpha-isomer, increased the firing probability, reduced the spike latency and enhanced the EPSP amplitude in response to synaptic activation. The effect was similar to the changes seen in long term potentiation. After alpha PDBu addition it was possible to elicit further enhancement by tetanization, but not after beta PDBu administration. (3) A slowly developing hyperpolarization was seen after active phorbol ester application without apparent changes in the soma input resistance. (4) Active phorbol esters reduced the slow afterhyperpolarization (AHP) in these cells without affecting the intermediate AHP.

MeSH Terms
Action Potentials/drug effects Animals Electric Stimulation Hippocampus/drug effects,physiology In Vitro Techniques Male Membrane Potentials/drug effects Phorbol 12,13-Dibutyrate Phorbol Esters/pharmacology Rats Rats, Inbred Strains Reaction Time/drug effects Synapses/drug effects,physiology Synaptic Transmission/drug effects
Chemicals
Phorbol Esters Phorbol 12,13-Dibutyrate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hvalby O
Institute of Neurophysiology, University of Oslo, Norway.
Reymann K
Andersen P
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Article Info
Journal
Experimental brain research
Abbr.
Exp Brain Res
ISSN
0014-4819
DOI
10.1007/BF00248751
Published
1988-00-00
Pages
588-96
Language
English
Region
Germany
NLM ID
0043312
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00248751 View journal details
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