Home LiteratureArticle Details
PMID: 2843568 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Platelet-derived growth factor mRNA detection in human atherosclerotic plaques by in situ hybridization.

The Journal of clinical investigation ·Vol. 82 ·No. 3 ·1988-09-00 ·Pages 1134-43

Wilcox JN, Smith KM, Williams LT, Schwartz SM, Gordon D

Abstract

Platelet-derived growth factor (PDGF) mRNA, and mRNA for its receptor, have been localized to specific cell types within the human atherosclerotic plaque, using in situ hybridization. The predominant cell types found to express PDGF A and B chain mRNA are mesenchymal-appearing intimal cells and endothelial cells, respectively, with little or no expression detected in macrophages. The distribution of PDGF receptor mRNA containing cells was also examined and found to be localized predominantly in the plaque intima.

MeSH Terms
Arteriosclerosis/genetics,metabolism,pathology Autoradiography Carotid Arteries Endothelium, Vascular/analysis,metabolism,pathology Humans Nucleic Acid Hybridization Platelet-Derived Growth Factor/analysis,genetics,metabolism RNA, Messenger/analysis,metabolism Receptors, Cell Surface/analysis Receptors, Platelet-Derived Growth Factor
Chemicals
Platelet-Derived Growth Factor RNA, Messenger Receptors, Cell Surface Receptors, Platelet-Derived Growth Factor
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wilcox J N
Department of Cardiovascular Research, Genentech, Inc., South San Francisco, California 94080.
Smith K M
Williams L T
Schwartz S M
Gordon D
References (60)
60 references, click to expand
  1. Atheroma: the artery wall and the environment.
    Int Rev Exp Pathol. 1980;21:55-118 PMID: 6993391
  2. Human atherosclerosis. I. Cell constitution and characteristics of advanced lesions of the superficial femoral artery.
    Am J Pathol. 1984 Jan;114(1):79-93 PMID: 6691417
  3. Heterogeneity of intermediate filament expression in vascular smooth muscle: a gradient in desmin positive cells from the rat aortic arch to the level of the arteria iliaca communis.
    Differentiation. 1981;20(3):196-202 PMID: 7040147
  4. Autoradiographic studies of the smooth muscle cells in human arteries.
    Paroi Arterielle. 1981;7(3):107-12 PMID: 7339541
  5. Platelet-derived growth factor: identification of constituent polypeptide chains.
    Biochem Biophys Res Commun. 1982 Jan 15;104(1):66-74 PMID: 7073684
  6. Ulex europaeus I lectin as a marker for vascular endothelium in human tissues.
    Lab Invest. 1982 Jul;47(1):60-6 PMID: 6177923
  7. Alterations in vascular smooth muscle mass in the spontaneously hypertensive rat. Role of cellular hypertrophy, hyperploidy, and hyperplasia.
    Circ Res. 1982 Sep;51(3):280-9 PMID: 7116579
  8. Chromosomal mapping of the simian sarcoma virus onc gene analogue in human cells.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4691-5 PMID: 6289313
  9. Platelet-derived growth factor is a chemoattractant for vascular smooth muscle cells.
    J Cell Physiol. 1982 Nov;113(2):261-6 PMID: 6184376
  10. Ulex europaeus I lectin as a marker for tumors derived from endothelial cells.
    Am J Clin Pathol. 1983 Jan;79(1):32-6 PMID: 6401381
  11. Cell proliferation in normal and atherosclerotic human aorta. I. Flow cytofluorometric determination of cellular deoxyribonucleic acid content.
    Lab Invest. 1983 Apr;48(4):395-8 PMID: 6834785
  12. Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor.
    Science. 1983 Jul 15;221(4607):275-7 PMID: 6304883
  13. Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus.
    Nature. 1983 Jul 7-13;304(5921):35-9 PMID: 6306471
  14. Autoradiographic and ultrastructural studies on the human fibro-atheromatous plaque.
    Atherosclerosis. 1983 Jul;48(1):95-100 PMID: 6882512
  15. A significant part of macrophage-derived growth factor consists of at least two forms of PDGF.
    Cell. 1985 Nov;43(1):277-86 PMID: 2416458
  16. Kinetics of cellular proliferation after arterial injury. I. Smooth muscle growth in the absence of endothelium.
    Lab Invest. 1983 Sep;49(3):327-33 PMID: 6887785
  17. Localization of cells synthesizing transforming growth factor-alpha mRNA in the mouse brain.
    J Neurosci. 1988 Jun;8(6):1901-4 PMID: 2455024
  18. Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes.
    Dev Biol. 1984 Feb;101(2):485-502 PMID: 6692991
  19. Cultured endothelial cells produce multiple growth factors for connective tissue cells.
    Exp Cell Res. 1984 Jul;153(1):167-72 PMID: 6376150
  20. Structure of a human smooth muscle actin gene (aortic type) with a unique intron site.
    Mol Cell Biol. 1984 Jun;4(6):1073-8 PMID: 6330528
  21. Transforming potential of human c-sis nucleotide sequences encoding platelet-derived growth factor.
    Science. 1984 Aug 10;225(4662):636-9 PMID: 6740330
  22. Human transforming growth factor-alpha: precursor structure and expression in E. coli.
    Cell. 1984 Aug;38(1):287-97 PMID: 6088071
  23. Release of endothelial cell-derived growth factor (ECDGF) by heparin.
    J Cell Physiol. 1984 Oct;121(1):13-21 PMID: 6480707
  24. Developmentally regulated production of platelet-derived growth factor-like molecules.
    Nature. 1984 Oct 18-24;311(5987):669-71 PMID: 6090952
  25. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  26. Platelet-derived growth factor.
    Mol Cell Endocrinol. 1985 Mar;39(3):169-87 PMID: 2984061
  27. Identification of foam cells in human atherosclerotic lesions as macrophages using monoclonal antibodies.
    Arch Pathol Lab Med. 1985 May;109(5):445-9 PMID: 2580504
  28. Modulation of the sis gene transcript during endothelial cell differentiation in vitro.
    Science. 1985 May 17;228(4701):882-5 PMID: 3890179
  29. Cytoskeletal features of rat aortic cells during development. An electron microscopic, immunohistochemical, and biochemical study.
    Circ Res. 1985 Jun;56(6):829-38 PMID: 3891126
  30. Arterial smooth muscle cells in primary culture produce a platelet-derived growth factor-like protein.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4418-22 PMID: 2989820
  31. Smooth muscle phenotypic expression in human carotid arteries. I. Comparison of cells from diffuse intimal thickenings adjacent to atheromatous plaques with those of the media.
    Lab Invest. 1985 Nov;53(5):556-62 PMID: 4057952
  32. Symposium on in vitro studies related to atherogenesis. Life histories of hyperplastoid cell lines from aorta and skin.
    Exp Mol Pathol. 1973 Apr;18(2):125-41 PMID: 4634042
  33. Evidence for a monoclonal origin of human atherosclerotic plaques.
    Proc Natl Acad Sci U S A. 1973 Jun;70(6):1753-6 PMID: 4515934
  34. The pathogenesis of atherosclerosis (first of two parts).
    N Engl J Med. 1976 Aug 12;295(7):369-77 PMID: 819830
  35. The pathogenesis of atherosclerosis (second of two parts).
    N Engl J Med. 1976 Aug 19;295(8):420-5 PMID: 778621
  36. Platelet interaction with damaged rabbit aorta.
    Lab Invest. 1979 Feb;40(2):194-200 PMID: 431038
  37. The evolution of vascular changes in the spontaneously hypertensive rat.
    Am J Pathol. 1980 Feb;98(2):357-84 PMID: 7355987
  38. Effects of hypertension and its reversal on aortic intima lesions of the rat.
    Hypertension. 1980 Jan-Feb;2(1):33-44 PMID: 7372354
  39. The pathogenesis of atherosclerosis--an update.
    N Engl J Med. 1986 Feb 20;314(8):488-500 PMID: 3511384
  40. Kinetics of cellular proliferation after arterial injury. III. Endothelial and smooth muscle growth in chronically denuded vessels.
    Lab Invest. 1986 Mar;54(3):295-303 PMID: 3512908
  41. Vasoconstriction: a new activity for platelet-derived growth factor.
    Science. 1986 Apr 4;232(4746):87-90 PMID: 3485309
  42. Regional accumulations of T cells, macrophages, and smooth muscle cells in the human atherosclerotic plaque.
    Arteriosclerosis. 1986 Mar-Apr;6(2):131-8 PMID: 2937395
  43. Replication of smooth muscle cells in vascular disease.
    Circ Res. 1986 Apr;58(4):427-44 PMID: 3516443
  44. cDNA sequence and chromosomal localization of human platelet-derived growth factor A-chain and its expression in tumour cell lines.
    Nature. 1986 Apr 24-30;320(6064):695-9 PMID: 3754619
  45. Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human alveolar macrophages.
    J Clin Invest. 1986 Jul;78(1):61-6 PMID: 3722386
  46. Fine structure of human aortic intimal thickening and fatty streaks.
    Lab Invest. 1965 Oct;14(10):1764-83 PMID: 5842381
  47. Full-length von Willebrand factor (vWF) cDNA encodes a highly repetitive protein considerably larger than the mature vWF subunit.
    EMBO J. 1986 Aug;5(8):1839-47 PMID: 3019665
  48. Production of platelet-derived growth factor-like molecules by cultured arterial smooth muscle cells accompanies proliferation after arterial injury.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7311-5 PMID: 3020543
  49. Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions.
    Am J Pathol. 1986 Oct;125(1):191-207 PMID: 3777135
  50. The hypogonadal mouse: reproductive functions restored by gene therapy.
    Science. 1986 Dec 12;234(4782):1372-8 PMID: 3097822
  51. A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation.
    J Cell Biol. 1986 Dec;103(6 Pt 2):2787-96 PMID: 3539945
  52. Morphology and cell kinetics of fatty streak lesion formation in the hypercholesterolemic rabbit.
    Am J Pathol. 1986 Dec;125(3):450-9 PMID: 3799815
  53. HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization.
    Am J Pathol. 1987 Jan;126(1):51-60 PMID: 3544852
  54. sis (platelet-derived growth factor B chain) gene transcript levels are elevated in human atherosclerotic lesions compared to normal artery.
    Proc Natl Acad Sci U S A. 1987 Feb;84(4):1099-103 PMID: 2434949
  55. Exaggerated spontaneous release of platelet-derived growth factor by alveolar macrophages from patients with idiopathic pulmonary fibrosis.
    N Engl J Med. 1987 Jul 23;317(4):202-9 PMID: 3600711
  56. Production and auto-induction of transforming growth factor-alpha in human keratinocytes.
    Nature. 1987 Aug 27-Sep 2;328(6133):817-20 PMID: 2442615
  57. Regulated expression of the platelet-derived growth factor A chain gene in microvascular endothelial cells.
    J Biol Chem. 1987 Oct 25;262(30):14381-4 PMID: 3478335
  58. Primary structure and mRNA localization of protein F1, a growth-related protein kinase C substrate associated with synaptic plasticity.
    EMBO J. 1987 Dec 1;6(12):3641-6 PMID: 3428269
  59. Platelet-derived growth factor gene expression in human atherosclerotic plaques and normal artery wall.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2810-4 PMID: 3282240
  60. Smooth muscle cell hypertrophy versus hyperplasia in hypertension.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7759-63 PMID: 6950415
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113671
Published
1988-09-00
Pages
1134-43
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC303629
Subset
IM
Grants
NHLBI NIH HHS · P01-HL-03174 · United States
NHLBI NIH HHS · R01 HL-32898 · United States
External Links
PubMed source DOI: 10.1172/JCI113671 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2