Home LiteratureArticle Details
PMID: 2843671 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Use of simian virus 40 replication to amplify Epstein-Barr virus shuttle vectors in human cells.

Journal of virology ·Vol. 62 ·No. 10 ·1988-10-00 ·Pages 3738-46

Heinzel SS, Krysan PJ, Calos MP, DuBridge RB

Abstract

We have increased the copy number of Epstein-Barr virus vectors that also carry the origin of replication of simian virus 40 (SV40) by providing a transient dose of SV40 T antigen. T antigen was supplied in trans by transfection of a nonreplicating plasmid which expresses T antigen into cells carrying Epstein-Barr virus-SV40 vectors. A significant increase in vector copy number occurred over the next few days. We also observed a high frequency of intramolecular recombination when the vector carried a repeat segment in direct orientation, but not when the repeat was in inverted orientation or absent. Furthermore, by following the mutation frequency for a marker on the vector after induction of SV40 replication, it was determined that SV40 replication generates a detectable increase in the deletion frequency but no measurable increase in the frequency of point mutations.

MeSH Terms
Antigens, Polyomavirus Transforming/genetics Cell Line DNA Replication DNA, Viral/biosynthesis,genetics Densitometry Gene Amplification Genetic Vectors Herpesvirus 4, Human/genetics,physiology Humans Mutation Nucleic Acid Hybridization Plasmids Simian virus 40/genetics,physiology Transfection Virus Replication
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Heinzel S S
Department of Genetics, Stanford University School of Medicine, California 94305.
Krysan P J
Calos M P
DuBridge R B
References (35)
35 references, click to expand
  1. Stable replication of plasmids derived from Epstein-Barr virus in various mammalian cells.
    Nature. 1985 Feb 28-Mar 6;313(6005):812-5 PMID: 2983224
  2. Amplification of a bovine papillomavirus-simian virus 40 chimera.
    J Virol. 1985 Nov;56(2):625-7 PMID: 2997482
  3. Chemically induced mutagenesis in a shuttle vector with a low-background mutant frequency.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3402-6 PMID: 3010297
  4. Negative control of DNA replication in composite SV40-bovine papilloma virus plasmids.
    Cell. 1986 Aug 29;46(5):741-52 PMID: 3017566
  5. Repression of bovine papilloma virus replication is mediated by a virally encoded trans-acting factor.
    Cell. 1986 Aug 29;46(5):753-62 PMID: 3017567
  6. A putative origin of replication of plasmids derived from Epstein-Barr virus is composed of two cis-acting components.
    Mol Cell Biol. 1985 Aug;5(8):1822-32 PMID: 3018528
  7. Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.
    Mol Cell Biol. 1985 Aug;5(8):2051-60 PMID: 3018548
  8. Alu-Alu recombination deletes splice acceptor sites and produces secreted low density lipoprotein receptor in a subject with familial hypercholesterolemia.
    J Biol Chem. 1987 Mar 5;262(7):3354-61 PMID: 3818645
  9. Electroporation for the efficient transfection of mammalian cells with DNA.
    Nucleic Acids Res. 1987 Feb 11;15(3):1311-26 PMID: 3029703
  10. Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system.
    Mol Cell Biol. 1987 Jan;7(1):379-87 PMID: 3031469
  11. Replication of latent Epstein-Barr virus genomes in Raji cells.
    J Virol. 1987 May;61(5):1743-6 PMID: 3033303
  12. Expression and rescuing of a cloned human tumour necrosis factor gene using an EBV-based shuttle cosmid vector.
    EMBO J. 1987 Feb;6(2):355-61 PMID: 3034585
  13. Recombinational substrates designed to study recombination between unique and repetitive sequences in vivo.
    Proc Natl Acad Sci U S A. 1987 Sep;84(17):6215-9 PMID: 3306671
  14. Cis-acting negative control of DNA replication in eukaryotic cells.
    Cell. 1988 Feb 12;52(3):397-404 PMID: 2830984
  15. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  16. Replication of the resident repressed Epstein-Barr virus genome during the early S phase (S-1 period) of nonproducer Raji cells.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):631-3 PMID: 4362625
  17. Fate of polyoma form IDNA during replication.
    J Virol. 1975 Jul;16(1):70-4 PMID: 166209
  18. Characteristics of a human cell line transformed by DNA from human adenovirus type 5.
    J Gen Virol. 1977 Jul;36(1):59-74 PMID: 886304
  19. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.
    Cell. 1978 Jul;14(3):725-31 PMID: 210957
  20. Evolutionary variants of simian virus 40: Cellular DNA sequences and sequences at recombinant joints of substituted variants.
    J Mol Biol. 1978 Dec 5;126(2):275-88 PMID: 216811
  21. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  22. SV40-transformed simian cells support the replication of early SV40 mutants.
    Cell. 1981 Jan;23(1):175-82 PMID: 6260373
  23. On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions.
    Cell. 1982 Jun;29(2):319-28 PMID: 6288254
  24. Persistence of freely replicating SV40 recombinant molecules carrying a selectable marker in permissive simian cells.
    Cell. 1982 Sep;30(2):499-508 PMID: 6291772
  25. Preparation of a "functional library" of African green monkey DNA fragments which substitute for the processing/polyadenylation signal in the herpes simplex virus type 1 thymidine kinase gene.
    Mol Cell Biol. 1983 Apr;3(4):643-53 PMID: 6304500
  26. Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3010-4 PMID: 6304690
  27. High mutation frequency in DNA transfected into mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3015-9 PMID: 6574469
  28. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  29. A cis-acting element from the Epstein-Barr viral genome that permits stable replication of recombinant plasmids in latently infected cells.
    Proc Natl Acad Sci U S A. 1984 Jun;81(12):3806-10 PMID: 6328526
  30. Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication.
    EMBO J. 1984 Jun;3(6):1243-53 PMID: 6086311
  31. DNA sequence and expression of the B95-8 Epstein-Barr virus genome.
    Nature. 1984 Jul 19-25;310(5974):207-11 PMID: 6087149
  32. Transfected DNA is mutated in monkey, mouse, and human cells.
    Mol Cell Biol. 1984 Oct;4(10):1951-60 PMID: 6095032
  33. A mammalian host-vector system that regulates expression and amplification of transfected genes by temperature induction.
    Science. 1985 Jan 4;227(4682):23-8 PMID: 2981116
  34. High spontaneous mutation frequency in shuttle vector sequences recovered from mammalian cellular DNA.
    Mol Cell Biol. 1984 Nov;4(11):2266-72 PMID: 6096690
  35. A vector that replicates as a plasmid and can be efficiently selected in B-lymphoblasts transformed by Epstein-Barr virus.
    Mol Cell Biol. 1985 Feb;5(2):410-3 PMID: 2983194
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.10.3738-3746.1988
Published
1988-10-00
Pages
3738-46
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253517
Subset
IM
Grants
NCI NIH HHS · 5T 32 CA 09302 · United States
NCI NIH HHS · CA 33056 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.10.3738-3746.1988 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2