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PMID: 2845417 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cloning and characterization of a cDNA encoding the chloroplastic copper/zinc-superoxide dismutase from pea.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 20 ·1988-10-00 ·Pages 7661-5

Scioli JR, Zilinskas BA

Abstract

A cDNA encoding the chloroplastic copper/zinc-superoxide dismutase of pea (Pisum sativum L.) was isolated from a cDNA library constructed in lambda gt11 from leaf mRNA. Nucleotide sequence analysis of the 875-base-pair clone revealed that it contained the complete coding sequence of the mature superoxide dismutase isozyme subunit, along with sequence information for a 48-amino acid N-terminal transit peptide. The deduced amino acid sequence of the mature subunit proved to be 64-87% homologous with amino acid sequences of copper/zinc-superoxide dismutases from other plant species. In vitro transcription, followed by cell-free translation, of the cDNA resulted in the formation of a 23.5-kDa precursor polypeptide, which, upon incubation with isolated pea chloroplasts, was imported and processed to its mature subunit molecular mass of 17.4 kDa.

MeSH Terms
Amino Acid Sequence Base Sequence Brassica Chloroplasts/enzymology Cloning, Molecular DNA/genetics DNA Restriction Enzymes Electrophoresis, Polyacrylamide Gel Fabaceae Isoenzymes/genetics Molecular Sequence Data Plants/enzymology,genetics Plants, Medicinal Protein Biosynthesis Superoxide Dismutase/genetics Transcription, Genetic Vegetables Zea mays
Chemicals
Isoenzymes DNA Superoxide Dismutase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Scioli J R
Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, NJ 08903.
Zilinskas B A
References (26)
26 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. In vitro synthesis, importation and processing of Mn-superoxide dismutase (SOD-3) into maize mitochondria.
    Biochim Biophys Acta. 1987 Oct 8;926(1):16-25 PMID: 2443181
  3. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4 PMID: 4525168
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Subcellular localisation and identification of superoxide dismutase in the leaves of higher plants.
    Eur J Biochem. 1978 Nov 15;91(2):339-44 PMID: 729573
  6. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  7. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  8. Absence of the iron-containing superoxide dismutase in mitochondria from mustard (Brassica campestris).
    Biochem J. 1981 Apr 1;195(1):229-33 PMID: 7306048
  9. Biosynthesis of mitochondrial manganese superoxide dismutase in saccharomyces cerevisiae. Precursor form of mitochondrial superoxide dismutase made in the cytoplasm.
    J Biol Chem. 1982 Mar 10;257(5):2713-8 PMID: 7037771
  10. The primary structure of Cu-Zn superoxide dismutase from Photobacterium leiognathi: evidence for a separate evolution of Cu-Zn superoxide dismutase in bacteria.
    Hoppe Seylers Z Physiol Chem. 1983 Jun;364(6):675-90 PMID: 6884993
  11. A novel chromatographic procedure for purification of bacterial plasmids.
    Anal Biochem. 1983 Jul 1;132(1):219-24 PMID: 6312836
  12. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  13. Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blots.
    J Cell Biol. 1984 Jul;99(1 Pt 1):20-8 PMID: 6203917
  14. Nucleotide sequence analysis of the nuclear gene coding for manganese superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron-sulphur protein.
    Eur J Biochem. 1985 Feb 15;147(1):153-61 PMID: 3882422
  15. In vitro transcription of chloroplast protein genes.
    Methods Enzymol. 1986;118:232-53 PMID: 2419733
  16. Dideoxy sequencing method using denatured plasmid templates.
    Anal Biochem. 1986 Feb 1;152(2):232-8 PMID: 3516005
  17. Isolation of nuclear encoded plastid ribosomal protein cDNAs.
    Mol Gen Genet. 1986 Feb;202(2):186-93 PMID: 3517591
  18. Amino acid sequence of copper,zinc-superoxide dismutase from spinach leaves.
    J Biochem. 1986 May;99(5):1289-98 PMID: 3519601
  19. The transport of proteins into chloroplasts.
    Annu Rev Biochem. 1986;55:879-912 PMID: 3527053
  20. Primary structure of Cu-Zn superoxide dismutase of Brassica oleracea proves homology with corresponding enzymes of animals, fungi and prokaryotes.
    Biol Chem Hoppe Seyler. 1986 Oct;367(10):1007-16 PMID: 3790249
  21. Cloning of cDNA for maize superoxide dismutase 2 (SOD2).
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):179-83 PMID: 3467349
  22. Protein import into chloroplasts requires a chloroplast ATPase.
    Proc Natl Acad Sci U S A. 1987 May;84(10):3288-92 PMID: 2953027
  23. Selection of AUG initiation codons differs in plants and animals.
    EMBO J. 1987 Jan;6(1):43-8 PMID: 3556162
  24. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  25. Isolation and sequence of complementary DNA encoding human extracellular superoxide dismutase.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6340-4 PMID: 3476950
  26. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.
    Anal Biochem. 1971 Nov;44(1):276-87 PMID: 4943714
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.20.7661
Published
1988-10-00
Pages
7661-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282252
Subset
IM
Databases
GENBANK
J04087
External Links
PubMed source DOI: 10.1073/pnas.85.20.7661 PMC Full Text View journal details
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