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PMID: 2845861 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cloning of nisin resistance determinant and replication origin on 7.6-kilobase EcoRI fragment of pNP40 from Streptococcus lactis subsp. diacetylactis DRC3.

Applied and environmental microbiology ·Vol. 54 ·No. 8 ·1988-08-00 ·Pages 2136-9

Froseth BR, Herman RE, McKay LL

Abstract

The nisin resistance determinant and an origin of replication on pNP40, a plasmid of about 60 kilobases that is present in Streptococcus lactis subsp. diacetylactis DRC3, was cloned on a 7.6-kilobase EcoRI fragment. When self-ligated, this fragment existed as an independent replicon (pFM011) and contained a 2.6-kilobase EcoRI-XbaI fragment encoding nisin resistance.

MeSH Terms

Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/genetics Deoxyribonuclease EcoRI Drug Resistance, Microbial Genes, Bacterial Genetic Markers Lactococcus lactis/drug effects,genetics Nisin/pharmacology R Factors Replicon

Chemicals

DNA, Bacterial Genetic Markers Nisin DNA Restriction Enzymes Deoxyribonuclease EcoRI

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Froseth B R

Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108.

Herman R E

McKay L L

References (9)

9 references, click to expand

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A rapid boiling method for the preparation of bacterial plasmids.
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Partial purification, specificity and mechanism of action of the nisin-inactivating enzyme from Bacillus cereus.
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Simple and rapid method for isolating large plasmid DNA from lactic streptococci.
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Streptococcus-Escherichia coli shuttle vector pSA3 and its use in the cloning of streptococcal genes.
Appl Environ Microbiol. 1985 Jan;49(1):115-9 PMID: 3883896
Transfer of Sucrose-Fermenting Ability and Nisin Production Phenotype among Lactic Streptococci.
Appl Environ Microbiol. 1985 Mar;49(3):627-33 PMID: 16346756
Plasmid transformation of Streptococcus lactis protoplasts: optimization and use in molecular cloning.
Appl Environ Microbiol. 1984 Aug;48(2):252-9 PMID: 6091544
Conjugative 40-megadalton plasmid in Streptococcus lactis subsp. diacetylactis DRC3 is associated with resistance to nisin and bacteriophage.
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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.54.8.2136-2139.1988

Published

1988-08-00

Pages

2136-9

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC202817

Subset

External Links

PubMed source DOI: 10.1128/aem.54.8.2136-2139.1988 PMC Full Text View journal details

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Cloning of nisin resistance determinant and replication origin on 7.6-kilobase EcoRI fragment of pNP40 from Streptococcus lactis subsp. diacetylactis DRC3.

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