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PMID: 2851729 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Generation of murine stromal cell lines supporting hematopoietic stem cell proliferation by use of recombinant retrovirus vectors encoding simian virus 40 large T antigen.

Molecular and cellular biology ·Vol. 8 ·No. 9 ·1988-09-00 ·Pages 3864-71

Williams DA, Rosenblatt MF, Beier DR, Cone RD

Abstract

The bone marrow is a complex microenvironment made up of multiple cell types which appears to play an important role in the maintenance of hematopoietic stem cell self-renewal and proliferation. We used murine long-term marrow cultures and a defective recombinant retrovirus vector containing the simian virus 40 large T antigen to immortalize marrow stromal cells which can support hematopoiesis in vitro for up to 5 weeks. Such cloned cell lines differentially supported stem cells which, when transplanted, allowed survival of lethally irradiated mice, formed hematopoietic spleen colonies in vivo, and stimulated lymphocyte proliferation in vitro. Molecular and functional analyses of these cell lines did not demonstrate the production of any growth factors known to support the proliferation of primitive hematopoietic stem cells. All cell lines examined produced macrophage colony-stimulating factor. The use of immortalizing retrovirus vectors may allow determination of unique cellular proteins important in hematopoietic stem cell proliferation by the systematic comparison of stromal cells derived from a variety of murine tissues.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/genetics Blotting, Southern Bone Marrow Cells Cell Division Cell Line Cell Transformation, Neoplastic Cells, Cultured Clone Cells DNA/genetics Genetic Vectors Growth Substances/analysis Hematopoiesis Hematopoietic Stem Cells/cytology Male Mice Mice, Inbred C3H Simian virus 40/genetics
Chemicals
Antigens, Polyomavirus Transforming Growth Substances DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Williams D A
Division of Hematology-Oncology, Children's Hospital, Boston, Massachusetts.
Rosenblatt M F
Beier D R
Cone R D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.9.3864-3871.1988
Published
1988-09-00
Pages
3864-71
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365445
Subset
IM
Grants
NHLBI NIH HHS · 2P01-HL32262 · United States
NHLBI NIH HHS · 5H08-HL-0554 · United States
NCI NIH HHS · CA13106 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.9.3864-3871.1988 PMC Full Text View journal details
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