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PMID: 2859593 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 9 ·1985-05-00 ·Pages 2834-8

Wells D, Kedes L

Abstract

We have isolated and sequenced full-length cDNA clones encoding the human basally expressed H3.3 histone from a human fibroblast cDNA library. Several features of this atypical cDNA distinguish it and its gene from the well-characterized cell-cycle regulated histone genes and their RNA transcripts. The H3.3 mRNA is approximately equal to 1200 bases long, contains unusually long 5' and 3' untranslated regions, and has a 3' polyadenylylated terminus. In addition, we have isolated and characterized a cDNA clone that is a precursor to the H3.3 mRNA and contains an intervening sequence interrupting its 5' untranslated region. Hybridization of subsegments of the cDNA to human genomic DNA reveals a complex multigene family. The differences in the structures of basal and cell-cycle histone genes suggest a model to explain the differences in their expression.

MeSH Terms
Base Sequence Cell Cycle Cloning, Molecular DNA/genetics Gene Amplification Gene Expression Regulation Genes Genetic Variation Histones/genetics Humans Poly A/genetics RNA, Messenger/genetics Transcription, Genetic
Chemicals
Histones RNA, Messenger Poly A DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wells D
Kedes L
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.9.2834
Published
1985-05-00
Pages
2834-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397660
Subset
IM
Grants
NIGMS NIH HHS · GM17995 · United States
Databases
GENBANK
M11353, M11354
External Links
PubMed source DOI: 10.1073/pnas.82.9.2834 PMC Full Text View journal details
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