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PMID: 6457299 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation of two clusters of mouse histone genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 7 ·1981-07-00 ·Pages 4078-82

Sittman DB, Chiu IM, Pan CJ, Cohn RH, Kedes LH, Marzluff WF

Abstract

Histone mRNA was partially purified from mouse myeloma cells synchronized in S phase by isoleucine starvation. A cDNA was prepared that contained sequences complementary to all five mouse histone genes. This cDNA was used to screen a library of mouse DNA in lambda phage. The positive clones were screened by hybridization with sea urchin histone gene-specific probes to identify those clones that contained histone genes. Confirmation of this identification was obtained by hybridization with Drosophila histone genes. Two independent clusters of histone genes were isolated. One, MM531, contains regions hybridizing specifically to H3, H4, and H1 and the other, MM221, contains two regions hybridizing specifically to H3 and single regions complementary to H4, H2b, and H2a. They are not part of a simple repeating structure. The nucleotide sequence of the coding region of the H3 gene in MM531 has been determined. This gene could code for a variant H3 protein that has several amino acid substitutions not reported in other H3 proteins.

MeSH Terms
Animals Bacteriophage lambda Cells, Cultured Chromosome Mapping Cloning, Molecular Genes Genetic Linkage Histones/genetics Mice/genetics
Chemicals
Histones
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sittman D B
Chiu I M
Pan C J
Cohn R H
Kedes L H
Marzluff W F
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.7.4078
Published
1981-07-00
Pages
4078-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319729
Subset
IM
Grants
NIA NIH HHS · AG-00143 · United States
NCI NIH HHS · CA 00178 · United States
NIGMS NIH HHS · GM 07158 · United States
Databases
GENBANK
J00421
External Links
PubMed source DOI: 10.1073/pnas.78.7.4078 PMC Full Text View journal details
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