MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain.

The EMBO journal ·Vol. 4 ·No. 1 ·1985-01-00 ·Pages 113-8

Clark MJ, Gagnon J, Williams AF, Barclay AN

Abstract

The MRC OX-2 antigen is a rat cell surface glycoprotein of mol. wt. 41 000-47 000 found on neurones, thymocytes, B cells, follicular dendritic cells and endothelium. We now report the amino sequence for this antigen as deduced from the nucleotide sequence of cDNA clones detected by use of an oligonucleotide probe. The sequence contains 248 amino acid residues of which 202 residues are likely to be outside the cell with two domains that show homology with immunoglobulins. The N-terminal domain fits best with Ig V domains and Thy-1 antigen while the C-terminal part is like an Ig C domain. Thus the structure overall is similar to an Ig light chain or the T cell receptor beta chain. Three glycosylation sites are identified on each of the MRC OX-2 antigen domains.

MeSH Terms

Amino Acid Sequence Animals Antigens, Surface/genetics Base Sequence Biological Evolution DNA/genetics Glycoproteins/genetics Immunoglobulin Light Chains/genetics Lymphocytes/immunology Membrane Proteins/genetics Neurons/immunology Rats Thy-1 Antigens

Chemicals

Antigens, Surface Glycoproteins Immunoglobulin Light Chains Membrane Proteins Thy-1 Antigens DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Clark M J

Gagnon J

Williams A F

Barclay A N

References (29)

29 references, click to expand

RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Three-dimensional structure of immunoglobulins.
Annu Rev Biochem. 1979;48:961-97 PMID: 89832
Identification of Ia glycoproteins in rat thymus and purification from rat spleen.
Eur J Immunol. 1979 Jun;9(6):426-33 PMID: 315315
Filter replicas and permanent collections of recombinant DNA plasmids.
Nucleic Acids Res. 1979 Dec 20;7(8):2115-36 PMID: 118435
Sequencing end-labeled DNA with base-specific chemical cleavages.
Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
The immunoglobulin mu chains of membrane-bound and secreted IgM molecules differ in their C-terminal segments.
Cell. 1980 Sep;21(2):393-406 PMID: 6773668
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.
Nucleic Acids Res. 1980 Mar 11;8(5):1081-96 PMID: 7443540
A rapid and efficient procedure for the purification of DNA from agarose gels.
Anal Biochem. 1980 Aug;106(2):492-6 PMID: 6255823
The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules.
Cell. 1981 May;24(2):287-99 PMID: 7016338
Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region.
Biochem J. 1981 Apr 1;195(1):15-30 PMID: 6118137
Analysis of structural similarities between brain Thy-1 antigen and immunoglobulin domains. Evidence for an evolutionary relationship and a hypothesis for its functional significance.
Biochem J. 1981 Apr 1;195(1):31-40 PMID: 6118138
Different reticular elements in rat lymphoid tissue identified by localization of Ia, Thy-1 and MRC OX 2 antigens.
Immunology. 1981 Dec;44(4):727-36 PMID: 6119291
Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin.
Science. 1982 May 14;216(4547):696-703 PMID: 6177036
Surface molecules and cell interactions.
J Theor Biol. 1982 Sep 21;98(2):221-34 PMID: 6184575
Purification and chemical characterisation of membrane glycoproteins from rat thymocytes and brain, recognised by monoclonal antibody MRC OX 2.
Eur J Biochem. 1982 Dec 15;129(2):447-58 PMID: 6129975
Patterns of amino acids near signal-sequence cleavage sites.
Eur J Biochem. 1983 Jun 1;133(1):17-21 PMID: 6852022
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
New M13 vectors for cloning.
Methods Enzymol. 1983;101:20-78 PMID: 6310323
The structure of the mouse immunoglobulin in gamma 3 membrane gene segment.
Nucleic Acids Res. 1983 Oct 11;11(19):6775-85 PMID: 6314258
The class II molecules of the human and murine major histocompatibility complex.
Cell. 1984 Jan;36(1):1-13 PMID: 6198089
The receptor for transepithelial transport of IgA and IgM contains multiple immunoglobulin-like domains.
Nature. 1984 Mar 1-7;308(5954):37-43 PMID: 6322002
Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins.
Nature. 1984 Mar 8-14;308(5955):153-8 PMID: 6546606
The structural basis of the multiple forms of human complement component C4.
Cell. 1984 Apr;36(4):907-14 PMID: 6546707
The gene structure of human anti-haemophilic factor IX.
EMBO J. 1984 May;3(5):1053-60 PMID: 6329734
Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences.
Nature. 1984 Jun 28-Jul 4;309(5971):757-62 PMID: 6330561
Compilation of published signal sequences.
Nucleic Acids Res. 1984 Jul 11;12(13):5145-64 PMID: 6379599
Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.
J Neurochem. 1984 Oct;43(4):1061-7 PMID: 6147390

Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1985-01-00

Pages

113-8

Language

English

Region

England

NLM ID

8208664

PMCID

PMC554158

Subset

Databases

GENBANK

X01785

External Links

PubMed source PMC Full Text View journal details

Analysis Services

MRC OX-2 antigen: a lymphoid/neuronal membrane glycoprotein with a structure like a single immunoglobulin light chain.

Contact

WeChat Official Account