Home LiteratureArticle Details
PMID: 2863737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Colonization factor antigen II (CFA/II) of enterotoxigenic Escherichia coli: molecular cloning of the CS3 determinant.

Molecular & general genetics : MGG ·Vol. 200 ·No. 2 ·1985-00-00 ·Pages 322-7

Manning PA, Timmis KN, Stevenson G

Abstract

The genes for the cell surface associated antigen CS3, produced by CFA/II type enterotoxigenic Escherichia coli, have been cloned in the plasmid vector pBR322 to produce a family of recombinant plasmids. These plasmids contain a series of HindIII fragments of which a fragment of 4.6 kb is common to all those expressing CS3. One of these plasmids, pPM474, has been subjected to mutagenesis with Tn1725 and deletions generated using Bal31. This has defined a minimum region of 3.75 kb necessary for the production of CS3 on the cell surface and implying genetic complexity as has been observed with other fimbrial antigens. Analysis of the plasmid encoded proteins in E. coli K-12 minicells has confirmed this complexity.

MeSH Terms
Antigens, Bacterial/genetics Chromosome Deletion Cloning, Molecular DNA Restriction Enzymes Escherichia coli/genetics,immunology,pathogenicity Fimbriae Proteins Fimbriae, Bacterial/ultrastructure Genes Genes, Bacterial Plasmids Virulence
Chemicals
Antigens, Bacterial colonization factor antigens Fimbriae Proteins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Manning P A
Timmis K N
Stevenson G
References (28)
28 references, click to expand
  1. New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8.
    Infect Immun. 1978 Aug;21(2):638-47 PMID: 80383
  2. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  3. Export without proteolytic processing of inner and outer membrane proteins encoded by F sex factor tra cistrons in Escherichia coli minicells.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):4837-41 PMID: 388418
  4. Characterization of new hydrophobic pili of human enterotoxigenic Escherichia coli: a possible new colonization factor.
    Infect Immun. 1984 Mar;43(3):959-65 PMID: 6199307
  5. Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans.
    Infect Immun. 1975 Sep;12(3):656-67 PMID: 1100526
  6. A simple method for the preparation of large quantities of pure plasmid DNA.
    Biochim Biophys Acta. 1975 Apr 2;383(4):457-63 PMID: 1092355
  7. Coli surface antigens 1 and 3 of colonization factor antigen II-positive enterotoxigenic Escherichia coli: morphology, purification, and immune responses in humans.
    Infect Immun. 1984 May;44(2):409-20 PMID: 6370866
  8. Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhea.
    Infect Immun. 1978 Feb;19(2):727-36 PMID: 344221
  9. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  10. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  11. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
    Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
  12. New fimbrial antigenic type (E8775) that may represent a colonization factor in enterotoxigenic Escherichia coli in humans.
    Infect Immun. 1982 Mar;35(3):1119-24 PMID: 6121756
  13. Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor.
    Infect Immun. 1977 Nov;18(2):330-7 PMID: 336541
  14. Rapid procedure for detection and isolation of large and small plasmids.
    J Bacteriol. 1981 Mar;145(3):1365-73 PMID: 7009583
  15. Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains.
    Microbiol Rev. 1982 Jun;46(2):129-61 PMID: 6126799
  16. A dot-immunobinding assay for monoclonal and other antibodies.
    Anal Biochem. 1982 Jan 1;119(1):142-7 PMID: 7072935
  17. Tn1721-encoded tetracycline resistance: mapping of structural and regulatory genes mediating resistance.
    J Bacteriol. 1983 Jan;153(1):116-23 PMID: 6294046
  18. A new colonization factor antigen (CFA/III) produced by enteropathogenic Escherichia coli O128:B12.
    Ann Microbiol (Paris). 1983 Jan-Feb;134A(1):53-64 PMID: 6342499
  19. Cloning of the structural gene (hly) for the haemolysin of Vibrio cholerae El Tor strain 017.
    Gene. 1984 Nov;31(1-3):225-31 PMID: 6098527
  20. Episome-carried surface antigen K88 of Escherichia coli. II. Isolation and chemical analysis.
    J Bacteriol. 1967 Feb;93(2):731-9 PMID: 4960185
  21. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.
    FEBS Lett. 1975 Oct 15;58(1):254-8 PMID: 773686
  22. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  23. Conjugation proteins encoded by the F sex factor.
    Nature. 1977 Dec 15;270(5638):580-5 PMID: 339109
  24. Two mannose-resistant haemagglutinins on enterotoxigenic Escherichia coli of serotype O6:K15:H16 or H-isolated from travellers' and infantile diarrhoea.
    J Gen Microbiol. 1982 Sep;128(9):2081-96 PMID: 6816903
  25. Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans.
    Infect Immun. 1982 Apr;36(1):189-97 PMID: 7042570
  26. Radioimmunological screening method for specific membrane proteins.
    Anal Biochem. 1979 Aug;97(1):153-7 PMID: 384844
  27. Expression of plasmids coding for colonization factor antigen II (CFA/II) and enterotoxin production in Escherichia coli.
    J Gen Microbiol. 1983 Dec;129(12):3591-601 PMID: 6142082
  28. Location of an F-pilin pool in the inner membrane.
    J Bacteriol. 1981 Apr;146(1):251-9 PMID: 6111549
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425443
Published
1985-00-00
Pages
322-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425443
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2