Home LiteratureArticle Details

PMID: 7042570 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans.

Infection and immunity ·Vol. 36 ·No. 1 ·1982-04-00 ·Pages 189-97

Cravioto A, Scotland SM, Rowe B

Abstract

We examined 205 enterotoxigenic strains of Escherichia coli for colonization factor antigens (CFA) I and II, using an immunodiffusion technique with specific antisera. A total of 36 strains of serogroups O63, O78, O114, O128, and O153 and 1 rough strain possessed CFA/I and gave a single precipitin line; 47 strains of serogroups O6, O8, O80, and O115 possessed CFA/II. The latter strains gave a major precipitin line (component 3) when tested with specific antisera prepared against strain E1392 or PB-176 (both E. coli O6.H16; biotype A). However, all 16 strains of E. coli O6.H16 belonging to biotype A gave a second precipitin line (component 1) when tested with both antisera. When CFA/II-positive strains were tested with a specific antiserum prepared against E. coli O6.H16 strains of biotype B or C, all strains gave component 3, but 16 of 17 strains of E. coli O6.H16 belonging to biotype B, C, or F gave a second precipitin line (component 2) not given by strains of biotype A. CFA/II-positive strains of serogroups other than O6 gave only component 3 in tests with all specific antisera. Nine enterotoxigenic strains of serotypes O7, O15, O25, O115, and O128 gave mannose-resistant hemagglutination of human or calf erythrocytes but lacked CFA/I or CFA/II. Although mannose-resistant hemagglutination was common in non-enterotoxigenic strains of E. coli, none of the non-enterotoxigenic strains possessed CFA/I or CFA/II; these strains included fecal strains of serogroups O6, O8, O63, and O78, fecal strains of enteropathogenic serogroups, and strains from extraintestinal sources.

MeSH Terms

Animals Antigens, Bacterial/analysis Cattle Enterotoxins/biosynthesis Escherichia coli/classification,immunology,metabolism Fimbriae Proteins Hemagglutination Humans Mannose/pharmacology Serotyping

Chemicals

Antigens, Bacterial Enterotoxins colonization factor antigens Fimbriae Proteins Mannose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cravioto A

Scotland S M

Rowe B

References (27)

27 references, click to expand

Test for Escherichia coli enterotoxin using infant mice: application in a study of diarrhea in children in Honolulu.
J Infect Dis. 1972 Apr;125(4):407-11 PMID: 4553083
Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture.
Science. 1974 Jan 25;183(4122):334-6 PMID: 4587905
Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.
Infect Immun. 1974 Aug;10(2):320-7 PMID: 4368545
Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans.
Infect Immun. 1975 Sep;12(3):656-67 PMID: 1100526
Special O:K:H serotypes among enterotoxigenic E. coli strains from diarrhea in adults and children. Occurrence of the CF (colonization factor) antigen and of hemagglutinating abilities.
Med Microbiol Immunol. 1977 Jul 18;163(2):99-110 PMID: 331065
Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor.
Infect Immun. 1977 Nov;18(2):330-7 PMID: 336541
Serotype-related enterotoxigenicity in Escherichia coli O6.H16 and O148.H28.
J Hyg (Lond). 1977 Dec;79(3):395-403 PMID: 336791
Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhea.
Infect Immun. 1978 Feb;19(2):727-36 PMID: 344221
Outbreak of infantile enteritis caused by enterotoxigenic Escherichia coli O6.H16.
J Clin Pathol. 1978 Mar;31(3):217-9 PMID: 346609
Role of Escherichia coli colonisation factor antigen in acute diarrhoea.
Lancet. 1978 Jul 22;2(8082):181-4 PMID: 78384
Association of hemolysin production, hemagglutination of human erythrocytes, and virulence for chicken embryos of extraintestinal Escherichia coli isolates.
Infect Immun. 1978 Apr;20(1):50-4 PMID: 352937
New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8.
Infect Immun. 1978 Aug;21(2):638-47 PMID: 80383
Relationship of hemagglutination to other biological properties of serologically classified isolates of Escherichia coli.
Infect Immun. 1979 Aug;25(2):507-12 PMID: 385499
The role of Escherichia coli in gastroenteritis.
Clin Gastroenterol. 1979 Sep;8(3):625-44 PMID: 387299
Hemagglutination by Escherichia coli in septicemia and urinary tract infections.
J Clin Microbiol. 1979 Oct;10(4):477-81 PMID: 393716
Production of K99 antigen by enterotoxigenic Escherichia coli strains of antigen groups o8, o9, o20, and o101 grown at different conditions.
Infect Immun. 1980 Jan;27(1):216-21 PMID: 6987169
Hemagglutination and colonization factors in enterotoxigenic and enteropathogenic Escherichia coli that cause diarrhea.
J Infect Dis. 1980 Jun;141(6):733-7 PMID: 6104683
Transfer of a CFA/I-ST plasmid promoted by a conjugative plasmid in a strain of Escherichia coli of serotype O128ac:H12.
Infect Immun. 1980 Jul;29(1):140-3 PMID: 6249752
Plasmids coding for heat-labile enterotoxin production isolated from Escherichia coli O78: comparison of properties.
J Bacteriol. 1980 Jul;143(1):158-67 PMID: 6995426
Molecular comparison of plasmids encoding heat-labile enterotoxin isolated from Escherichia coli strains of human origin.
J Bacteriol. 1980 Jul;143(1):168-75 PMID: 6249787
Analysis of parameters affecting the hemagglutination activity of Escherichia coli possessing colonization factor antigens: improved medium for observing erythrocyte agglutination.
J Clin Microbiol. 1981 Feb;13(2):301-8 PMID: 7009637
Hemagglutination of human type O erythrocytes, hemolysin production, and serogrouping of Escherichia coli isolates from patients with acute pyelonephritis, cystitis, and asymptomatic bacteriuria.
Infect Immun. 1981 Jan;31(1):309-15 PMID: 7011993
Production of heat-labile or heat-stable enterotoxins by strains of Escherichia coli belonging to serogroups O44, O114, and O128.
Infect Immun. 1981 Jan;31(1):500-3 PMID: 7012006
Hemagglutination typing of Escherichia coli: definition of seven hemagglutination types.
J Clin Microbiol. 1980 Aug;12(2):235-42 PMID: 7014607
Plasmids coding for colonization factor antigen I and heat-stable enterotoxin production isolated from enterotoxigenic Escherichia coli: comparison of their properties.
Infect Immun. 1981 May;32(2):927-36 PMID: 7019082
Serotypes of attachment pili of enterotoxigenic Escherichia coli isolated from humans.
Infect Immun. 1981 Jun;32(3):1254-60 PMID: 6114039
New fimbrial antigenic type (E8775) that may represent a colonization factor in enterotoxigenic Escherichia coli in humans.
Infect Immun. 1982 Mar;35(3):1119-24 PMID: 6121756

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.36.1.189-197.1982

Published

1982-04-00

Pages

189-97

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC351202

Subset

External Links

PubMed source DOI: 10.1128/iai.36.1.189-197.1982 PMC Full Text View journal details

Analysis Services

Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains of Escherichia coli isolated from humans.

Contact

WeChat Official Account