Phenotypic properties of a unique rpoA mutation (phs) of Escherichia coli.

Journal of bacteriology ·Vol. 164 ·No. 2 ·1985-11-00 ·Pages 904-10

Giffard PM, Rowland GC, Kroll RG, Stewart LM, Bakker EP, Booth IR

Abstract

The phs mutation of Escherichia coli has been suggested to affect the Na+/H+ antiport (D. Zilberstein, E. Padan, and S. Schuldiner, FEBS Lett. 168:327-330, 1980). We have recently shown that the mutation affects the rpoA gene and thus affects transcription. The extent of the pleiotropy of the phs mutation was investigated. In addition to the previously reported growth defect on L-glutamate and melibiose, the mutation also affects at least two other metabolic systems. The transport and metabolism of arabinose is impaired and the transport of sulfate is reduced. The extent to which the effects of the phs mutation on metabolism are due to a defect in the Na+/H+ antiport was investigated, and no causal role for this transport system in the metabolic defects was found.

MeSH Terms

Arabinose/metabolism Biological Transport Carrier Proteins/metabolism Cysteine/biosynthesis DNA-Directed RNA Polymerases/genetics Escherichia coli/enzymology,genetics,growth & development,metabolism Genes, Bacterial Glutamates/metabolism Glutamic Acid Melibiose/metabolism Mutation Phenotype Potassium/metabolism Proline/metabolism Sodium/metabolism,pharmacology Sodium-Hydrogen Exchangers Sulfates/metabolism

Chemicals

Carrier Proteins Glutamates Sodium-Hydrogen Exchangers Sulfates Glutamic Acid Melibiose Proline Sodium Arabinose DNA-Directed RNA Polymerases Cysteine Potassium

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Giffard P M

Rowland G C

Kroll R G

Stewart L M

Bakker E P

Booth I R

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26 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.164.2.904-910.1985

Published

1985-11-00

Pages

904-10

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC214337

Subset

External Links

PubMed source DOI: 10.1128/jb.164.2.904-910.1985 PMC Full Text View journal details

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Phenotypic properties of a unique rpoA mutation (phs) of Escherichia coli.

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