Molecular cloning and the nucleotide sequence of cDNA for neuron-specific enolase messenger RNA of rat brain.
The cDNAs to mRNA for rat gamma gamma enolase (neuron-specific enolase; NSE; EC 4.2.1.11) were isolated from a cDNA library by using differential colony hybridization and a hybrid-selected translation assay. By overlapping of the nucleotide sequences of several cDNA inserts, it was found that they spanned 2232 base pairs (bp) which included 1299 bp of the complete coding region, 68 bp of the 5' noncoding region, and 848 bp of the 3' noncoding region, including a polyadenylylation signal. In addition, the poly(A) tail was also found. The amino acid sequence deduced from the nucleotide sequence was composed of 433 amino acids. Southern blot analysis with a cDNA insert detected one hybridizing fragment in rat genomic DNA digested with several different restriction enzymes. Dot-blot and transfer hybridization analyses of poly(A)+ RNA from developing rat brains showed an increase of NSE mRNA 10-30 days after birth.
16 references, click to expand
-
Improved estimation of secondary structure in ribonucleic acids.Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
-
The nucleotide sequence of a rat 18 S ribosomal ribonucleic acid gene and a proposal for the secondary structure of 18 S ribosomal ribonucleic acid.J Biol Chem. 1984 Jan 10;259(1):224-30 PMID: 6323401
-
The primary structure of rabbit beta-globin mRNA as determined from cloned DNA.Cell. 1977 Apr;10(4):571-85 PMID: 558827
-
Conservation of the primary structure at the 3' end of 18S rRNA from eucaryotic cells.Cell. 1978 Mar;13(3):551-63 PMID: 77738
-
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
-
Biosynthesis of the brain-specific 14-3-2 protein in a cell-free system from wheat germ extract directed with poly(A)-containing RNA from rat brain.J Neurochem. 1980 Mar;34(3):687-93 PMID: 7354341
-
Sequencing end-labeled DNA with base-specific chemical cleavages.Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
-
Plasmid screening at high colony density.Gene. 1980 Jun;10(1):63-7 PMID: 6997135
-
Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.Proc Natl Acad Sci U S A. 1981 Apr;78(4):2253-7 PMID: 6166005
-
5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency.Nucleic Acids Res. 1981 May 25;9(10):2251-66 PMID: 6166921
-
Purification of messenger RNA coding for rat liver aldolase B subunit.Biochem Biophys Res Commun. 1981 May 15;100(1):407-12 PMID: 6167260
-
Partial purification and characterization of messenger RNA coding 14-3-2 protein from rat brain.J Neurochem. 1982 Aug;39(2):366-70 PMID: 6177835
-
Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.J Biol Chem. 1982 Aug 10;257(15):8569-72 PMID: 7096324
-
Changes in levels of translatable mRNA for neuron-specific enolase and non-neuronal enolase during development of rat brain and liver.J Biochem. 1983 Nov;94(5):1443-50 PMID: 6654865
-
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
-
Action of crystalline acid carboxypeptidase from Penicillium janthinellum.Biochim Biophys Acta. 1975 Aug 26;397(2):443-8 PMID: 239751
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
