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PMID: 2866956 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proton conduction by subunit a of the membrane-bound ATP synthase of Escherichia coli revealed after induced overproduction.

The EMBO journal ·Vol. 4 ·No. 9 ·1985-09-00 ·Pages 2357-63

von Meyenburg K, Jørgensen BB, Michelsen O, Sørensen L, McCarthy JE

Abstract

Transcriptional fusions between the phage lambda promotor pR and ATP synthase genes, atp, on plasmid pBR322 were constructed in order to study the effects upon growth and physiology of Escherichia coli of induced overproduction of H+-ATPase subunits. Constitutive overproduction of the complete enzyme had earlier been found to result in decreased growth rate and cytological defects. When a 15-fold overproduction of subunit a alone, or together with subunit c, or with all other ATP synthase subunits was suddenly induced, the following effects were observed. Inhibition of growth and protein synthesis within 10 min of induction, which effect was suppressed by N,N'-dicyclohexylcarbodiimide, also when the chromosomal atp genes coding for the Fo subunits a, b and c were deleted. Partial collapse of the membrane potential delta psi at 4-6 min after induction paralleled by inhibition of thiomethylgalactoside and guanosine transport. Respiration and alpha-methylglucoside transport was not affected. The partial collapse of delta psi, and the specific inhibition of proton-driven transport systems is taken to show that the subunit a has--when suddenly overproduced and inserted into the membrane--a protonophoric activity. It is suggested that this protonophoric activity of subunit a is related to the function of this subunit in the Fo sector in H+-ATPases.

MeSH Terms
Bacteriophage lambda/genetics Cell Membrane/enzymology DNA Restriction Enzymes Escherichia coli/enzymology,genetics Genes Genes, Bacterial Genotype Kinetics Macromolecular Substances Plasmids Proton-Translocating ATPases/genetics,metabolism Transcription, Genetic
Chemicals
Macromolecular Substances DNA Restriction Enzymes Proton-Translocating ATPases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
von Meyenburg K
Jørgensen B B
Michelsen O
Sørensen L
McCarthy J E
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-09-00
Pages
2357-63
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554510
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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