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PMID: 6325392 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overproduction of subunit a of the F0 component of proton-translocating ATPase inhibits growth of Escherichia coli cells.

Journal of bacteriology ·Vol. 158 ·No. 1 ·1984-04-00 ·Pages 300-6

Kanazawa H, Kiyasu T, Noumi T, Futai M

Abstract

A hybrid plasmid, pKY159, carrying the promoter and the proximal region of the gene cluster for proton-translocating ATPase caused growth inhibition of Escherichia coli cells (K. Yamaguchi and M. Yamaguchi, J. Bacteriol. 153:550-554, 1983). The mechanism of this growth inhibition was studied, especially in terms of the responsible gene(s). Insertion of IS1, IS5, or gamma delta between the promoter and the gene for a possible component of the ATPase of 14,000 daltons (14K protein) released the inhibitory effect by pKY159. Deletion of the gene for subunit a also released the effect. However, deletion in the gene for the 14K protein released the effect only with an additional insertion within the gene. These results suggested that overproduction of subunit a is closely related to growth inhibition, whereas the 14K protein is not.

MeSH Terms
Chromosome Deletion DNA Transposable Elements Escherichia coli/enzymology,genetics,growth & development Gene Expression Regulation Genes, Bacterial Genetic Complementation Test Operon Plasmids Proton-Translocating ATPases/biosynthesis,genetics
Chemicals
DNA Transposable Elements Proton-Translocating ATPases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kanazawa H
Kiyasu T
Noumi T
Futai M
References (41)
41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.158.1.300-306.1984
Published
1984-04-00
Pages
300-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215412
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.158.1.300-306.1984 PMC Full Text View journal details
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