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PMID: 386330 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and mapping of plasmids containing the Salmonella typhimurium origin of DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3097-101

Zyskind JW, Deen LT, Smith DW

Abstract

A purified EcoRI restriction endonuclease fragment that determines resistance to kanamycin and is incapable of self-replication was used to select autonomously replicating fragments from an EcoRI digest of a Salmonella typhimurium F' plasmid containing the chromosomal region believed to include the S. typhimurium origin of DNA replication. Both the F factor and S. typhimurium chromosome replication origins were cloned by this procedure. The EcoRI fragmentment containing the S. typhimurium origin of replication is 19.4 kilobase pairs long and includes functional asp+ and uncB+ genes. Restriction endonuclease analysis of deletions obtained from the S. typhimurium origin plasmid indicated that the replication origin (ori region) is contained within a 3.3-kilobase pair region. Comparison with Escherichia coli origin plasmids shows colinearity of gene arrangement on the chromosomes in this region and suggests that some, but not all, regions of the nucleotide sequence in the origin region may be conserved (identical) in these two bacterial species.

MeSH Terms
Chromosome Mapping DNA Replication DNA Restriction Enzymes DNA, Bacterial/genetics Drug Resistance, Microbial Escherichia coli/genetics Plasmids Salmonella typhimurium/genetics Transformation, Bacterial
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zyskind J W
Deen L T
Smith D W
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3097
Published
1979-07-00
Pages
3097-101
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383770
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.7.3097 PMC Full Text View journal details
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