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PMID: 2869943 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Differences in mRNA levels in Anabaena living freely or in symbiotic association with Azolla.

The EMBO journal ·Vol. 5 ·No. 1 ·1986-01-00 ·Pages 29-35

Nierzwicki-Bauer SA, Haselkorn R

Abstract

Azolla is a small water fern in whose leaf cavities the filamentous nitrogen-fixing cyanobacterium Anabaena azollae is symbiotically associated. Using cloned genes from Anabaena 7120 for glutamine synthetase (GS), ribulose-1,5-bisphosphate (RuBP) carboxylase, nitrogenase and the 32-kd protein of photosystem II, mRNA levels of the corresponding genes in the Anabaena endosymbiont were studied by Northern hybridization. In RNA isolated from the endosymbiont there is a 10-fold reduction of GS transcript levels, a greater than 5-fold increase in 32-kd transcript levels and a greater than 5-fold decrease in RuBP carboxylase transcript levels, compared with levels in the free-living Anabaena azollae. In the endosymbiont and in heterocysts of the free-living Anabaena azollae the nif H, nif D, and nif K genes are transcribed from a single nif HDK operon.

MeSH Terms
Chlorophyll/genetics Cloning, Molecular Cyanobacteria/genetics,physiology Genes Glutamate-Ammonia Ligase/genetics Light-Harvesting Protein Complexes Nitrogenase/genetics Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Physiological Phenomena Plant Proteins/genetics RNA, Messenger/genetics,isolation & purification Ribulose-Bisphosphate Carboxylase/genetics Symbiosis Transcription, Genetic
Chemicals
Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins RNA, Messenger Chlorophyll Nitrogenase Ribulose-Bisphosphate Carboxylase Glutamate-Ammonia Ligase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nierzwicki-Bauer S A
Haselkorn R
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28 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-01-00
Pages
29-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166691
Subset
IM
Grants
NIGMS NIH HHS · GM 21823 · United States
External Links
PubMed source PMC Full Text View journal details
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