Further studies on the mode of action of the heme-controlled translational inhibitor: stimulating protein acts at level of binary complex formation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 5 ·1979-05-00 ·Pages 2163-4

de Haro C, Ochoa S

Abstract

Previous work has shown that (i) at physiological concentrations of eukaryotic initiation factor 2 (eIF-2), formation of the ternary complex eIF-2-GTP-Met-tRNAi, which precedes the assembly of a 40S initiation complex, requires the presence of eIF-2 stimulating protein (ESP) and (ii) the interaction of eIF-2 with ESP is blocked by the translational inhibitor which, in reticulocyte lysates, is activated in the absence of hemin. Present evidence indicates that formation of the ternary complex is preceded by formation of the binary complex eIF-2-GTP and that ESP acts at the level of binary complex formation.

MeSH Terms

Animals Guanosine Triphosphate/metabolism Heme/deficiency Peptide Chain Initiation, Translational Peptide Initiation Factors/metabolism RNA, Transfer/metabolism Rabbits Reticulocytes/metabolism

Chemicals

Peptide Initiation Factors Heme Guanosine Triphosphate RNA, Transfer

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

de Haro C

Ochoa S

References (13)

13 references, click to expand

Effect of hemin on site-specific phosphorylation of eukaryotic initiation factor 2.
Proc Natl Acad Sci U S A. 1978 Feb;75(2):789-93 PMID: 273242
Regulation of protein synthesis in reticulocyte lysates: phosphorylation of methionyl-tRNAf binding factor by protein kinase activity of translational inhibitor isolated from hemedeficient lysates.
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3112-6 PMID: 184460
Specificity of the protein kinase activity associated with the hemin-controlled repressor of rabbit reticulocyte.
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3078-82 PMID: 184458
Further studies on the mode of action of the heme-controlled translational inhibitor.
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1741-5 PMID: 377282
Mode of action of the hemin-controlled inhibitor of protein synthesis: studies with factors from rabbit reticulocytes.
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2713-6 PMID: 275839
Regulation of protein synthesis: activation by double-stranded RNA of a protein kinase that phosphorylates eukaryotic initiation factor 2.
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1121-5 PMID: 274704
Phosphorylation of eukaryotic protein synthesis initiation factors.
Proc Natl Acad Sci U S A. 1978 Jan;75(1):108-12 PMID: 272626
Phosphorylation of initiation factor elF-2 and the control of reticulocyte protein synthesis.
Cell. 1977 May;11(1):187-200 PMID: 559547
The mechanism of action of protein synthesis initiation factors from rabbit reticulocytes.
J Biol Chem. 1978 May 10;253(9):3078-87 PMID: 641056
Initiation of mammalian protein synthesis. II. The assembly of the initiation complex with purified initiation factors.
J Mol Biol. 1977 Nov;116(4):755-67 PMID: 592399
Additional evidence that the hemin-controlled translational repressor from rabbit reticulocytes is a protein kinase.
Biochem Biophys Res Commun. 1977 Jan 24;74(2):559-69 PMID: 836310
Soluble factors required for eukaryotic protein synthesis.
Annu Rev Biochem. 1976;45:191-216 PMID: 786149
Binding of MET-TRNAf and GTP to homogeneous initiation factor MP.
J Biol Chem. 1975 Dec 10;250(23):9076-82 PMID: 1104615

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.76.5.2163

Published

1979-05-00

Pages

2163-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC383557

Subset

External Links

PubMed source DOI: 10.1073/pnas.76.5.2163 PMC Full Text View journal details

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Further studies on the mode of action of the heme-controlled translational inhibitor: stimulating protein acts at level of binary complex formation.

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