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PMID: 2873571 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A microtubule-activated ATPase from sea urchin eggs, distinct from cytoplasmic dynein and kinesin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 13 ·1986-07-00 ·Pages 4799-803

Collins CA, Vallee RB

Abstract

We report an ATPase activity, present in sea urchin egg cytosol, that is activated by microtubules. The activity sediments at 10 S in sucrose gradients and is clearly distinct from activities at 12 S and 20 S due to cytoplasmic dynein. Potent activation of the ATPase is observed when endogenous egg tubulin is induced to assemble with taxol or when exogenous taxol-stabilized pure brain tubulin microtubules or flagellar outer-doublet microtubules are added. No activation by tubulin subunits or taxol alone is detectable. In contrast to flagellar or cytoplasmic dynein, the microtubule-activated enzyme is unaffected by vanadate or by nonionic detergents and hydrolyzes GTP in addition to ATP. In contrast to kinesin, it cosediments with microtubules in the presence or absence of ATP. The microtubule-activated enzyme may have a role in microtubule-based motility.

MeSH Terms
Adenosine Triphosphatases/metabolism Alkaloids/pharmacology Animals Cytosol/enzymology Dyneins/metabolism Enzyme Activation GTP Phosphohydrolases/metabolism Kinesins Microtubule Proteins/metabolism Microtubules/enzymology Nerve Tissue Proteins/metabolism Ovum/enzymology Paclitaxel Sea Urchins/enzymology Substrate Specificity Tubulin/metabolism
Chemicals
Alkaloids Microtubule Proteins Nerve Tissue Proteins Tubulin Adenosine Triphosphatases GTP Phosphohydrolases Dyneins Kinesins Paclitaxel
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Collins C A
Vallee R B
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.13.4799
Published
1986-07-00
Pages
4799-803
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323829
Subset
IM
Grants
NIGMS NIH HHS · GM10111 · United States
NIGMS NIH HHS · GM32977 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.13.4799 PMC Full Text View journal details
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