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PMID: 2874480 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Neuroblastoma cells express c-sis and produce a transforming growth factor antigenically related to the platelet-derived growth factor.

Molecular and cellular biology ·Vol. 5 ·No. 9 ·1985-09-00 ·Pages 2289-97

van Zoelen EJ, van de Ven WJ, Franssen HJ, van Oostwaard TM, van der Saag PT, Heldin CH, de Laat SW

Abstract

Mouse neuroblastoma Neuro-2A cells produce transforming growth factors during exponential growth in a defined hormone-free medium, which, on Bio-Gel columns in 1 M HAc, elute at a molecular size of 15 to 20 kilodaltons (kDa). These neuroblastoma-derived transforming growth factors have strong mitogenic activity, but they do not compete with epidermal growth factor for receptor binding (E. J. J. van Zoelen, D. R. Twardzik, T. M. J. van Oostwaard, P. T. van der Saag, S. W. de Laat, and G. J. Todaro, Proc. Natl. Acad. Sci. U.S.A. 81:4085-4089, 1984). In this study approximately 80% of the mitogenic activity was immunoprecipitated by antibodies raised against platelet-derived growth factor (PDGF). Immunoblotting indicated a true molecular size of 32 kDa for this PDGF-like growth factor. Analysis of poly(A)+ RNA from Neuro-2A cells demonstrated the expression of the c-sis oncogene in this cell line, whereas in vitro translation of the RNA yielded a 20-kDa protein recognized by anti-PDGF antibodies. Separation by reverse-phase high-pressure liquid chromatography demonstrated the presence of two distinct mitogenic activities in neuroblastoma-derived transforming growth factor preparations, one of which is antigenically related to PDGF. Both activities had the ability to induce anchorage-independent growth in normal rat kidney cells, both in the presence and in the absence of epidermal growth factor. It is concluded that Neuro-2A cells express c-sis with concomitant production and secretion of a PDGF-like growth factor, which plays a role in the induction of phenotypic transformation on normal rat kidney cells.

MeSH Terms
Animals Cell Line Cell Transformation, Neoplastic Chromatography, High Pressure Liquid Epidermal Growth Factor/pharmacology Mice Neuroblastoma/genetics,metabolism Oncogene Proteins v-sis Peptide Biosynthesis Peptides/immunology,pharmacology Platelet-Derived Growth Factor/immunology Poly A/analysis,biosynthesis,genetics RNA, Messenger/analysis,biosynthesis,genetics Rats Retroviridae Proteins/biosynthesis,genetics Transforming Growth Factors
Chemicals
Oncogene Proteins v-sis Peptides Platelet-Derived Growth Factor RNA, Messenger Retroviridae Proteins Poly A Epidermal Growth Factor Transforming Growth Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
van Zoelen E J
van de Ven W J
Franssen H J
van Oostwaard T M
van der Saag P T
Heldin C H
de Laat S W
References (45)
45 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Platelet-derived growth factor agonist activity of a secreted form of the v-sis oncogene product.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1721-5 PMID: 2984666
  3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  4. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  5. A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels.
    Anal Biochem. 1979 Oct 1;98(2):305-9 PMID: 386835
  6. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  7. Chemical and biological properties of a growth factor from human-cultured osteosarcoma cells: resemblance with platelet-derived growth factor.
    J Cell Physiol. 1980 Nov;105(2):235-46 PMID: 6936400
  8. Similarities between fibroblast-derived growth factor and platelet-derived growth factor.
    Exp Cell Res. 1981 Sep;135(1):221-7 PMID: 6456922
  9. Demonstration of an antibody against platelet-derived growth factor.
    Exp Cell Res. 1981 Dec;136(2):255-61 PMID: 6171440
  10. Cellular genes analogous to retroviral onc genes are transcribed in human tumour cells.
    Nature. 1982 Jan 14;295(5845):116-9 PMID: 6173755
  11. Isolation from murine sarcoma cells of novel transforming growth factors potentiated by EGF.
    Nature. 1982 Feb 4;295(5848):417-9 PMID: 6276769
  12. Human transforming growth factor. Production by a melanoma cell line, purification, and initial characterization.
    J Biol Chem. 1982 May 10;257(9):5220-5 PMID: 6279663
  13. Platelet-derived growth factor: identification of constituent polypeptide chains.
    Biochem Biophys Res Commun. 1982 Jan 15;104(1):66-74 PMID: 7073684
  14. Expression of cellular homologues of retroviral onc genes in human hematopoietic cells.
    Proc Natl Acad Sci U S A. 1982 Apr;79(8):2490-4 PMID: 6283530
  15. Synergistic interaction of two classes of transforming growth factors from murine sarcoma cells.
    Cancer Res. 1982 Nov;42(11):4776-8 PMID: 6290046
  16. Blotting of RNA onto ion exchange paper allowing subsequent characterization by in situ translation in addition to blot hybridization.
    Nucleic Acids Res. 1982 Aug 25;10(16):4831-43 PMID: 7134000
  17. Purification by reverse-phase high-performance liquid chromatography of an epidermal growth factor-dependent transforming growth factor.
    Anal Biochem. 1982 Sep 1;125(1):217-24 PMID: 6983313
  18. Embryonal carcinoma cell growth and differentiation. Production of and response to molecules with transforming growth factor activity.
    Exp Cell Res. 1983 Jan;143(1):143-52 PMID: 6297938
  19. Transforming growth factors in solid human malignant neoplasms.
    Cancer Res. 1983 May;43(5):1966-71 PMID: 6299535
  20. Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization.
    J Biol Chem. 1983 Jun 10;258(11):7155-60 PMID: 6602130
  21. Simian sarcoma virus onc gene, v-sis, is derived from the gene (or genes) encoding a platelet-derived growth factor.
    Science. 1983 Jul 15;221(4607):275-7 PMID: 6304883
  22. Platelet-derived growth factor is structurally related to the putative transforming protein p28sis of simian sarcoma virus.
    Nature. 1983 Jul 7-13;304(5921):35-9 PMID: 6306471
  23. The molecular biology of platelet-derived growth factor.
    Cell. 1983 Jul;33(3):653-5 PMID: 6307524
  24. Cellular responsiveness to growth factors correlates with a cell's ability to express the transformed phenotype.
    Cell. 1983 Jul;33(3):931-8 PMID: 6307528
  25. Normal mouse serum contains peptides which induce fibroblasts to grow in soft agar.
    J Cell Biochem. 1983;21(1):29-38 PMID: 6603462
  26. Expression of a platelet-derived growth factor-like protein in simian sarcoma virus transformed cells.
    Science. 1983 Sep 30;221(4618):1348-50 PMID: 6310754
  27. Structure and activation of the human N-ras gene.
    Cell. 1983 Sep;34(2):581-6 PMID: 6616621
  28. Transforming growth factors released from Kirsten sarcoma virus transformed cells do not compete for epidermal growth factor membrane receptors.
    J Cell Physiol. 1983 Oct;117(1):116-22 PMID: 6311845
  29. Structural and immunological similarities between simian sarcoma virus gene product(s) and human platelet-derived growth factor.
    Nature. 1983 Oct 13-19;305(5935):605-8 PMID: 6312326
  30. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.
    Cell. 1983 Dec;35(3 Pt 2):603-10 PMID: 6606489
  31. Antisera to a synthetic peptide of the sis viral oncogene product recognize human platelet-derived growth factor.
    Nature. 1984 Jan 12-18;307(5947):180-3 PMID: 6197658
  32. Rat transforming growth factor type 1: structure and relation to epidermal growth factor.
    Science. 1984 Mar 9;223(4640):1079-82 PMID: 6320373
  33. A glioma-derived analog to platelet-derived growth factor: demonstration of receptor competing activity and immunological crossreactivity.
    Proc Natl Acad Sci U S A. 1984 Feb;81(3):926-30 PMID: 6322178
  34. Production of platelet-derived growth factor-like molecules and reduced expression of platelet-derived growth factor receptors accompany transformation by a wide spectrum of agents.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2396-400 PMID: 6326125
  35. Growth factors: mechanism of action and relation to oncogenes.
    Cell. 1984 May;37(1):9-20 PMID: 6373015
  36. Simian sarcoma virus--transformed cells secrete a mitogen identical to platelet-derived growth factor.
    Science. 1984 Jul 6;225(4657):54-6 PMID: 6328659
  37. The c-sis gene encodes a precursor of the B chain of platelet-derived growth factor.
    EMBO J. 1984 May;3(5):921-8 PMID: 6329745
  38. Cellular transformation by coordinated action of three peptide growth factors from human platelets.
    Nature. 1984 Jun 28-Jul 4;309(5971):804-6 PMID: 6330562
  39. Neuroblastoma cells produce transforming growth factors during exponential growth in a defined hormone-free medium.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4085-9 PMID: 6330739
  40. Enhanced expression of the human gene N-myc consequent to amplification of DNA may contribute to malignant progression of neuroblastoma.
    Proc Natl Acad Sci U S A. 1984 Aug;81(15):4940-4 PMID: 6589638
  41. A v-sis oncogene protein produced in bacteria competes for platelet-derived growth factor binding to its receptor.
    J Biol Chem. 1984 Sep 10;259(17):10645-8 PMID: 6088510
  42. Expression of cellular oncogenes during embryonic and fetal development of the mouse.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):7141-5 PMID: 6594688
  43. Coexpression of a PDGF-like growth factor and PDGF receptors in a human osteosarcoma cell line: implications for autocrine receptor activation.
    Cell. 1984 Dec;39(3 Pt 2):447-57 PMID: 6096004
  44. Phenotypic transformation of normal rat kidney cells in a growth-factor-defined medium: induction by a neuroblastoma-derived transforming growth factor independently of the EGF receptor.
    J Cell Physiol. 1985 May;123(2):151-60 PMID: 2984216
  45. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
    Biochemistry. 1974 Aug 13;13(17):3606-15 PMID: 4858491
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.9.2289-2297.1985
Published
1985-09-00
Pages
2289-97
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366955
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.5.9.2289-2297.1985 PMC Full Text View journal details
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