Blotting of RNA onto ion exchange paper allowing subsequent characterization by in situ translation in addition to blot hybridization.

Nucleic acids research ·Vol. 10 ·No. 16 ·1982-08-25 ·Pages 4831-43

Saris CJ, Franssen HJ, Heuyerjans JH, van Eenbergen J, Bloemers HP

Abstract

We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels. In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reproducible. Examples of applications are shown and possible other applications are discussed.

MeSH Terms

Animals Cell-Free System Chromatography, Ion Exchange/methods Moloney murine leukemia virus/genetics Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics RNA, Viral/genetics Reticulocytes/metabolism

Chemicals

RNA, Messenger RNA, Viral

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Saris C J

Franssen H J

Heuyerjans J H

van Eenbergen J

Bloemers H P

References (12)

12 references, click to expand

The synthesis of celluloses containing covalently bound nucleotides, polynucleotides, and nucleic acids.
Biochemistry. 1968 Aug;7(8):2809-13 PMID: 5666753
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
Biochemistry. 1974 Aug 13;13(17):3606-15 PMID: 4858491
Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct.
Biochemistry. 1975 Jan 14;14(1):69-78 PMID: 1109593
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
An efficient mRNA-dependent translation system from reticulocyte lysates.
Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: enhancement of the gag-pol polypeptide with yeast suppressor tRNA.
Cell. 1978 Jan;13(1):189-99 PMID: 202399
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
An improved method for the preparation of undegraded polysomes and active messenger RNA from immature chicken erythrocytes.
Anal Biochem. 1979 Oct 1;98(2):464-71 PMID: 496012
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
M-MuLV-induced leukemogenesis: integration and structure of recombinant proviruses in tumors.
Cell. 1981 Jun;24(3):729-39 PMID: 7249080
Expression of the PRC II avian sarcoma virus genome.
J Virol. 1982 Mar;41(3):767-80 PMID: 6284969

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.16.4831

Published

1982-08-25

Pages

4831-43

Language

English

Region

England

NLM ID

0411011

PMCID

PMC320821

Subset

External Links

PubMed source DOI: 10.1093/nar/10.16.4831 PMC Full Text View journal details

Analysis Services

Blotting of RNA onto ion exchange paper allowing subsequent characterization by in situ translation in addition to blot hybridization.

Contact

WeChat Official Account