Home LiteratureArticle Details
PMID: 2875030 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Receptor-binding function of type 1 pili effects bladder colonization by a clinical isolate of Escherichia coli.

Infection and immunity ·Vol. 53 ·No. 3 ·1986-09-00 ·Pages 693-6

Keith BR, Maurer L, Spears PA, Orndorff PE

Abstract

The role of type 1 pili in promoting bladder colonization was examined by constructing two mutant strains of a clinical Escherichia coli isolate. One mutant was isogenic to the parental strain save for a lesion in a gene required for pilus receptor binding; the other mutant was isogenic save for a lesion in the gene encoding the pilus structural subunit. Using mixed infections of the parental and mutant strains in an ascending rat cystitis model, we found that type 1-piliated mutants that lacked the receptor-binding function were as ineffective in bladder colonization as were mutants lacking the entire organelle.

MeSH Terms
Adhesiveness Animals Bacterial Vaccines/immunology Escherichia coli/pathogenicity Fimbriae, Bacterial/physiology Humans Mutation Rats Receptors, Immunologic/metabolism Urinary Bladder/microbiology
Chemicals
Bacterial Vaccines Receptors, Immunologic pili, bacterial receptor
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Keith B R
Maurer L
Spears P A
Orndorff P E
References (24)
24 references, click to expand
  1. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5.
    Gene. 1982 Oct;19(3):327-36 PMID: 6295884
  2. Non-flagellar appendages of bacteria.
    Nature. 1959 Mar 21;183(4664):782-6 PMID: 13644195
  3. Contribution of adhesion to bacterial persistence in the mouse urinary tract.
    Infect Immun. 1983 Apr;40(1):265-72 PMID: 6131870
  4. Role of type 1 fimbriae in the pathogenesis of ascending urinary tract infection induced by escherichia coli in mice.
    Infect Immun. 1983 Mar;39(3):1307-15 PMID: 6132876
  5. Mannose-sensitive and Gal-Gal binding Escherichia coli pili from recombinant strains. Chemical, functional, and serological properties.
    J Exp Med. 1983 Nov 1;158(5):1713-19 PMID: 6195290
  6. Mutations in E coli cistrons affecting adhesion to human cells do not abolish Pap pili fiber formation.
    EMBO J. 1984 May;3(5):1159-65 PMID: 6145589
  7. Genes of pyelonephritogenic E. coli required for digalactoside-specific agglutination of human cells.
    EMBO J. 1984 May;3(5):1167-73 PMID: 6145590
  8. Organization and expression of genes responsible for type 1 piliation in Escherichia coli.
    J Bacteriol. 1984 Aug;159(2):736-44 PMID: 6146599
  9. Nucleotide sequence of pilA, the gene encoding the structural component of type 1 pili in Escherichia coli.
    J Bacteriol. 1985 Apr;162(1):454-7 PMID: 2858471
  10. Adhesion to human cells by Escherichia coli lacking the major subunit of a digalactoside-specific pilus-adhesin.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1800-4 PMID: 2858852
  11. Protection against Escherichia coli-induced urinary tract infections with hybridoma antibodies directed against type 1 fimbriae or complementary D-mannose receptors.
    Infect Immun. 1985 Jun;48(3):625-8 PMID: 2860067
  12. Two modes of control of pilA, the gene encoding type 1 pilin in Escherichia coli.
    J Bacteriol. 1985 Oct;164(1):321-30 PMID: 3930469
  13. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  14. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  15. Escherichia coli pili as possible mediators of attachment to human urinary tract epithelial cells.
    Infect Immun. 1978 Jul;21(1):229-37 PMID: 361565
  16. Antipili antibody affords protection against experimental ascending pyelonephritis.
    J Clin Invest. 1979 Jul;64(1):333-6 PMID: 376560
  17. Prevention of colonization of the urinary tract of mice with Escherichia coli by blocking of bacterial adherence with methyl alpha-D-mannopyranoside.
    J Infect Dis. 1979 Mar;139(3):329-32 PMID: 376757
  18. Effect of piliation on Klebsiella pneumoniae infection in rat bladders.
    Infect Immun. 1980 Nov;30(2):554-61 PMID: 6108291
  19. Detection of enterotoxigenic Escherichia coli by DNA colony hybridization.
    J Infect Dis. 1980 Dec;142(6):892-8 PMID: 7007526
  20. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface.
    J Infect Dis. 1981 Mar;143(3):325-45 PMID: 7014727
  21. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate.
    Infect Immun. 1981 Sep;33(3):933-8 PMID: 6116675
  22. Haemolysin contributes to virulence of extra-intestinal E. coli infections.
    Nature. 1981 Dec 17;294(5842):665-7 PMID: 7031483
  23. F7 and type 1-like fimbriae from three Escherichia coli strains isolated from urinary tract infections: protein chemical and immunological aspects.
    Infect Immun. 1982 May;36(2):462-8 PMID: 6123483
  24. A pilus peptide vaccine for the prevention of gonorrhea.
    Prog Allergy. 1983;33:314-31 PMID: 6187017
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.53.3.693-696.1986
Published
1986-09-00
Pages
693-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260849
Subset
IM
Grants
NIAID NIH HHS · 1RO1 AI22223-01 · United States
External Links
PubMed source DOI: 10.1128/iai.53.3.693-696.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2