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PMID: 2876429 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Amino acid sequence of the plasma membrane ATPase of Neurospora crassa: deduction from genomic and cDNA sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 20 ·1986-10-00 ·Pages 7693-7

Hager KM, Mandala SM, Davenport JW, Speicher DW, Benz EJ, Slayman CW

Abstract

The plasma membrane of Neurospora crassa contains an electrogenic H+-ATPase (EC 3.6.1.35), for which we have isolated and sequenced both genomic and cDNA clones. The ATPase gene is interrupted by four small introns (58-124 base pairs). It encodes a protein of 920 amino acids (Mr, 99,886) possessing as many as eight transmembrane segments. The Neurospora ATPase shows significant amino acid sequence homology with the Na+,K+- and Ca2+-transporting ATPases of animal cells, particularly in regions that appear to be involved in ATP binding and hydrolysis.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Membrane/enzymology DNA/analysis Neurospora/enzymology Neurospora crassa/enzymology Proton-Translocating ATPases/analysis,genetics
Chemicals
DNA Proton-Translocating ATPases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hager K M
Mandala S M
Davenport J W
Speicher D W
Benz E J
Slayman C W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.20.7693
Published
1986-10-00
Pages
7693-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386787
Subset
IM
Grants
NIGMS NIH HHS · GM 07499 · United States
NIGMS NIH HHS · GM 10363 · United States
NIGMS NIH HHS · GM 15761 · United States
Databases
GENBANK
M14085
External Links
PubMed source DOI: 10.1073/pnas.83.20.7693 PMC Full Text View journal details
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