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PMID: 2877950 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Contribution of cloned virulence factors from uropathogenic Escherichia coli strains to nephropathogenicity in an experimental rat pyelonephritis model.

Infection and immunity ·Vol. 54 ·No. 3 ·1986-12-00 ·Pages 761-7

Marre R, Hacker J, Henkel W, Goebel W

Abstract

Escherichia coli 536 (O6:K15:H31), which was isolated from a case of urinary tract infection, determines high nephropathogenicity in a rat pyelonephritis system as measured by renal bacterial counts 7 days after infection. The loss of S fimbrial adhesin formation (Sfa-) (mannose-resistant hemagglutination [Mrh-] and fimbria production [Fim-]), serum resistance (Sre-), and hemolysin production (Hly-) in the mutant 536-21 led to a dramatic reduction of bacterial counts from almost 10(5) to only 40 cells per g of kidney. The reintroduction of the cloned S fimbrial adhesin determinant (sfa) increases the virulence of the avirulent mutant strain by a factor of 20; almost the same effect was observed after restoration of serum resistance by integration of an sfa+ recombinant cosmid into the chromosome. Additional reintroduction of the Hly+ phenotype by transformation of two hly determinants increased the virulence of the strains. Hemolysin production determined increased renal elimination of leukocytes and erythrocytes. Thus all three determinants investigated, S fimbriae, serum resistance, and hemolysin, contribute to the multifactorial phenomenon of E. coli nephropathogenicity.

MeSH Terms
Adhesins, Escherichia coli Animals Bacterial Adhesion Bacterial Proteins/genetics Blood Bactericidal Activity Cloning, Molecular Disease Models, Animal Escherichia coli/genetics,pathogenicity Fimbriae, Bacterial Hemagglutinins/genetics Hemolysin Proteins/genetics Pyelonephritis/microbiology Rats
Chemicals
Adhesins, Escherichia coli Bacterial Proteins Hemagglutinins Hemolysin Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Marre R
Hacker J
Henkel W
Goebel W
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39 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.54.3.761-767.1986
Published
1986-12-00
Pages
761-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260234
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.54.3.761-767.1986 PMC Full Text View journal details
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