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PMID: 2878919 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa.

Journal of bacteriology ·Vol. 169 ·No. 1 ·1987-01-00 ·Pages 33-41

Mattick JS, Bills MM, Anderson BJ, Dalrymple B, Mott MR, Egerton JR

Abstract

Type 4 fimbriae are found in a range of pathogenic bacteria, including Bacteroides nodosus, Moraxella bovis, Neisseria gonorrhoeae, and Pseudomonas aeruginosa. The structural subunits of these fimbriae all contain a highly conserved hydrophobic amino-terminal sequence preceding a variable hydrophilic carboxy-terminal region. We show here that recombinant P. aeruginosa cells containing the B. nodosus fimbrial subunit gene under the control of a strong promoter (pL, from bacteriophage lambda) produced large amounts of fimbriae that were structurally and antigenically indistinguishable from those produced by B. nodosus. This was demonstrated by fimbrial isolation and purification, electrophoretic and Western transfer analyses, and immunogold labeling and electron microscopy. These results suggest that type 4 fimbriated bacteria use a common mechanism for fimbrial assembly and that the structural subunits are interchangeable, thereby providing a basis for the development of multivalent vaccines.

MeSH Terms
Amino Acid Sequence Bacteroides/genetics,ultrastructure Electrophoresis, Polyacrylamide Gel Fimbriae, Bacterial/analysis Microscopy, Electron Moraxella/genetics,ultrastructure Morphogenesis Neisseria gonorrhoeae/genetics,ultrastructure Operon Plasmids Pseudomonas aeruginosa/genetics,ultrastructure
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mattick J S
Bills M M
Anderson B J
Dalrymple B
Mott M R
Egerton J R
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43 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.1.33-41.1987
Published
1987-01-00
Pages
33-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211730
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.169.1.33-41.1987 PMC Full Text View journal details
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